PRKCDBP Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PRKCDBP Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(26)

Kat-Nr. : 16250-1-AP

Synonyme

Caveolae-associated protein 3, Cavin 3, Cavin-3, hSRBC, Protein kinase C delta-binding protein

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  WB analysis of HeLa using 16250-1-AP

  WB result of PRKCDBP antibody (16250-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRKCDBP transfected HeLa cells.

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  WB analysis using 16250-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16250-1-AP (PRKCDBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 16250-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16250-1-AP (PRKCDBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 16250-1-AP

  IP result of anti-PRKCDBP (IP:16250-1-AP, 4ug; Detection:16250-1-AP 1:1000) with A549 cells lysate 1120 ug.

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  IHC staining of human colon using 16250-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 16250-1-AP (PRKCDBP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 16250-1-AP

  Immunofluorescent analysis of MCF-7 cells, using PRKCDBP antibody 16250-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, HeLa-Zellen, MCF-7-Zellen, Mauslungengewebe, Rattenlungengewebe
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: human normal colon; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 21 publications below
• IHC: See 4 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Produktinformation

16250-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PRKCDBP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRKCDBP fusion protein Ag9209
• Vollständiger Name: protein kinase C, delta binding protein
• Berechnetes Molekulargewicht: 261 aa, 28 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC011585
• Gene symbol: PRKCDBP
• Gene ID (NCBI): 112464
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein kinase C delta-binding protein (PRKCDBP) is a putative tumor suppressor in which alteration has been observed in several human cancers. It is a BRCA1-interacting protein, and may also be involved in DNA damage response and participate in BRCA1-mediated tumor suppression pathways. PKCdelta binds to the C-terminal half of PRKCDBP through the regulatory domain and phosphorylates it in vitro. In COS1 cells, the phosphorylation of over-expressed PRKCDBP is stimulated by 12-O-tetradecanoylphorbol-13-acetate and further enhanced by the over-expression of PKCdelta

Publikationen

Species	Application	Title
human	WB	ACS Nano; Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.; Authors - Zhiwei Liao
mouse	WB	PLoS Biol; Endocytic crosstalk: cavins, caveolins, and caveolae regulate clathrin-independent endocytosis.; Authors - Natasha Chaudhary
human	WB,IF	Nat Commun; Identification of intracellular cavin target proteins reveals cavin-PP1alpha interactions regulate apoptosis.; Authors - Kerrie-Ann McMahon ; KD Validated
human	WB,IP,IF	Dev Cell; Caveolae sense oxidative stress through membrane lipid peroxidation and cytosolic release of CAVIN1 to regulate NRF2; Authors - Yeping Wu
mouse	WB,IF	Elife; Cavin-3 dictates the balance between ERK and Akt signaling.; Authors - Victor J Hernandez
mouse	WB	Elife; Deciphering caveolar functions by syndapin III KO-mediated impairment of caveolar invagination.; Authors - Eric Seemann ; KO Validated