PRKCDBP Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PRKCDBP Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16250-1-PBS

Synonyme

Caveolae-associated protein 3, Cavin 3, Cavin-3, hSRBC, Protein kinase C delta-binding protein

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  WB analysis of HeLa using 16250-1-AP (same clone as 16250-1-PBS)

  WB result of PRKCDBP antibody (16250-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRKCDBP transfected HeLa cells. This data was developed using the same antibody clone with 16250-1-PBS in a different storage buffer formulation.

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  WB analysis using 16250-1-AP (same clone as 16250-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16250-1-AP (PRKCDBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16250-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 16250-1-AP (same clone as 16250-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16250-1-AP (PRKCDBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16250-1-PBS in a different storage buffer formulation.

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  IP experiment of A549 using 16250-1-AP (same clone as 16250-1-PBS)

  IP result of anti-PRKCDBP (IP:16250-1-AP, 4ug; Detection:16250-1-AP 1:1000) with A549 cells lysate 1120 ug. This data was developed using the same antibody clone with 16250-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 16250-1-AP (same clone as 16250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 16250-1-AP (PRKCDBP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16250-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 16250-1-AP (same clone as 16250-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using PRKCDBP antibody 16250-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 16250-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16250-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA PRKCDBP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRKCDBP fusion protein Ag9209
• Vollständiger Name: protein kinase C, delta binding protein
• Berechnetes Molekulargewicht: 261 aa, 28 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC011585
• Gene symbol: PRKCDBP
• Gene ID (NCBI): 112464
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein kinase C delta-binding protein (PRKCDBP) is a putative tumor suppressor in which alteration has been observed in several human cancers. It is a BRCA1-interacting protein, and may also be involved in DNA damage response and participate in BRCA1-mediated tumor suppression pathways. PKCdelta binds to the C-terminal half of PRKCDBP through the regulatory domain and phosphorylates it in vitro. In COS1 cells, the phosphorylation of over-expressed PRKCDBP is stimulated by 12-O-tetradecanoylphorbol-13-acetate and further enhanced by the over-expression of PKCdelta