PRKCQ-Specific Polyklonaler Antikörper

PRKCQ-Specific Polyklonal Antikörper für WB, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19982-1-AP

Synonyme

PRKCQ, EC:2.7.11.13, nPKC-theta, PRKCT, protein kinase C

---

• 

  WB analysis using 19982-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19982-1-AP (PRKCQ-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19982-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19982-1-AP (PRKCQ-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 19982-1-AP

  IP result of anti-PRKCQ-Specific (IP:19982-1-AP, 4ug; Detection:19982-1-AP 1:500) with K-562 cells lysate 1600 ug.

• 

  FC experiment of K-562 using 19982-1-AP

  1x10^6 K-562 cells were intracellularly stained with 0.2 ug PRKCQ-Specific Polyclonal antibody (19982-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, MOLT-4-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in FC (Intra): K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

19982-1-AP bindet in WB, FC (Intra), IP, ELISA PRKCQ-Specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: protein kinase C, theta
• Berechnetes Molekulargewicht: 82 kDa
• Beobachtetes Molekulargewicht: 72-74 kDa
• GenBank-Zugangsnummer: NM_006257
• Gene symbol: PRKCQ
• Gene ID (NCBI): 5588
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRKCQ, also named as PRKCT and nPKC-theta, belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and PKC subfamily. PRKCQ catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. PRKCQ is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. It is essential for T-cell receptor (TCR)-mediated T-cell activation, but it is dispensable during TCR-dependent thymocyte development. PRKCQ links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. It is required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. This antibody is specific to PRKCQ.