PRKCQ-Specific Polyklonaler Antikörper

PRKCQ-Specific Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19982-1-AP

Synonyme

PRKCQ, PRKCQ-Specific, PRKCT, Protein kinase C theta type, protein kinase C, theta

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Galerie der Validierungsdaten

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  WB analysis using 19982-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19982-1-AP (PRKCQ-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 19982-1-AP

  IP result of anti-PRKCQ-Specific(IP:19982-1-AP, 4ug; Detection:19982-1-AP 1:500) with Jurkat cells lysate 1400 ug.

• 

  IP experiment of K-562 using 19982-1-AP

  IP result of anti-PRKCQ-Specific(IP:19982-1-AP, 4ug; Detection:19982-1-AP 1:500) with K-562 cells lysate 1360 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen
• Erfolgreiche IP: Jurkat-Zellen, K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

19982-1-AP bindet in WB, IP, ELISA PRKCQ-Specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: protein kinase C, theta
• Berechnetes Molekulargewicht: 82 kDa
• Beobachtetes Molekulargewicht: 72-74 kDa
• GenBank-Zugangsnummer: NM_006257
• Gene symbol: PRKCQ
• Gene ID (NCBI): 5588
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRKCQ, also named as PRKCT and nPKC-theta, belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and PKC subfamily. PRKCQ catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. PRKCQ is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. It is essential for T-cell receptor (TCR)-mediated T-cell activation, but it is dispensable during TCR-dependent thymocyte development. PRKCQ links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. It is required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. This antibody is specific to PRKCQ.