DNA-PKcs Rekombinanter Antikörper

DNA-PKcs Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242676E6

Kat-Nr. : 85370-2-PBS

Synonyme

DNAPK, DNPK1, PRKDC, DNA-PK catalytic subunit, DNA-dependent protein kinase catalytic subunit

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  WB analysis of HT-29 using 85370-2-RR (same clone as 85370-2-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 85370-2-RR (DNA-PKcs antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85370-2-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 85370-2-RR (same clone as 85370-2-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 85370-2-RR (DNA-PKcs antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85370-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 85370-2-RR (same clone as 85370-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85370-2-RR (DNA-PKcs antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85370-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 85370-2-RR (same clone as 85370-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85370-2-RR (DNA-PKcs antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85370-2-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 85370-2-RR (same clone as 85370-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using DNA-PKcs antibody (85370-2-RR, Clone: 242676E6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85370-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 85370-2-RR (same clone as 85370-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DNA-PKcs antibody (85370-2-RR, Clone: 242676E6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85370-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 85370-2-RR (same clone as 85370-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DNA-PKcs antibody (85370-2-RR, Clone: 242676E6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85370-2-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 85370-2-RR (same clone as 85370-2-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug DNA-PKcs Recombinant antibody (85370-2-RR, Clone:242676E6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 85370-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

85370-2-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA DNA-PKcs und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: protein kinase, DNA-activated, catalytic polypeptide
• Berechnetes Molekulargewicht: 469 kDa
• Beobachtetes Molekulargewicht: 350-460 kDa
• GenBank-Zugangsnummer: NM_006904
• Gene symbol: PRKDC
• Gene ID (NCBI): 5591
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRKDC, also named as HYRC, HYRC1, DNPK1 and p460, belongs to the PI3/PI4-kinase family. PRKDC is a serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ), PRKDC is required for double-strand break (DSB) repair and V(D)J recombination. PRKDC must be bound to DNA to express its catalytic properties. It promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). It is required to protect and align broken ends of DNA. PRKDC may also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. It is found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. It also involved in modulation of transcription. It recognizes the substrate consensus sequence [ST]-Q. PRKDC phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism.