PRKG1 Polyklonaler Antikörper

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• KD/KO Validated

PRKG1 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(30)

Kat-Nr. : 21646-1-AP

Synonyme

CGKI, cGK1, PRKG1B, EC:2.7.11.12, cGMP-dependent protein kinase I

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  WB analysis using 21646-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21646-1-AP (PRKG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21646-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21646-1-AP (PRKG1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21646-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21646-1-AP (PRKG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 21646-1-AP

  IP result of anti-PRKG1 (IP:21646-1-AP, 4ug; Detection:21646-1-AP 1:600) with mouse brain tissue lysate 4000ug.

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  IHC staining of human heart using 21646-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21646-1-AP (PRKG1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 21646-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21646-1-AP (PRKG1 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Mausnierengewebe, Mauslungengewebe, Rattenhirngewebe, Rattenlungengewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 28 publications below
• IHC: See 7 publications below

Produktinformation

21646-1-AP bindet in WB, IHC, IP, ELISA PRKG1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRKG1 fusion protein Ag16333
• Vollständiger Name: protein kinase, cGMP-dependent, type I
• Berechnetes Molekulargewicht: 686 aa, 78 kDa
• Beobachtetes Molekulargewicht: 65-78 kDa
• GenBank-Zugangsnummer: BC127090
• Gene symbol: PRKG1
• Gene ID (NCBI): 5592
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRKG1(cGMP-dependent protein kinase 1) is also named as cGK1, PRKG1B, PRKGR1A, PRKGR1B and belongs to the cGMP subfamily. It serves as an integral component of second messenger signaling in a number of biological contexts including cell differentiation, memory, and vasodilation(PMID:21893290). Its activity prevents pathological-level nitric oxide-induced apoptosis and promotes DNA synthesis/cell proliferation in vascular smooth muscle cells(PMID:20060325). PRKG1 has 2 isoforms produced by alternative splicing with the molecular weight of 76 kDa and 78 kDa. The autophosphorylation can increase the kinase activity and it can form a monomer with the molecular weight of 65 kDa that is produced by proteolytic cleavage. SDS-PAGE revealed that proteolysis generated two different monomers with molecular masses of 70 and 68 kDa of CGK1-beta(PMID:8702828).

Publikationen

Species	Application	Title
human	IHC	Comput Struct Biotechnol J; Integrating single-cell sequencing and clinical insights to explore malignant transformation in odontogenic keratocyst; Authors - Guile Zhao
rat	WB	Environ Pollut; Fluoride-induced unrestored arrest during haploid period of spermatogenesis via the regulation of DDX25 in rats.; Authors - Yongli Han
mouse	WB	Clin Sci (Lond); Empagliflozin prevents cardiomyopathy via sGC-cGMP-PKG pathway in type 2 diabetes mice.; Authors - Mei Xue
bovine	WB	Front Cell Dev Biol; Myostatin Mutation Promotes Glycolysis by Increasing Phosphorylation of Phosphofructokinase via Activation of PDE5A-cGMP-PKG in Cattle Heart.; Authors - Mingjuan Gu ; KD Validated
rat	WB	Front Pharmacol; Emodin Interferes With Nitroglycerin-Induced Migraine in Rats Through CGMP-PKG Pathway.; Authors - Shuding Sun
human	WB	Cancer Cell Int; NEK7 promotes gastric cancer progression as a cell proliferation regulator.; Authors - Yi-Ke Li