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PRKG1 Rekombinanter Antikörper

PRKG1 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250348B5

Kat-Nr. : 85983-1-PBS

Synonyme

CGKI, cGK1, cGMP-dependent protein kinase 1, cGMP-dependent protein kinase I, EC:2.7.11.12

Filter:
  • Western Blot (WB) analysis of various lysates using PRKG1 Recombinant monoclonal antibody (85983-1-RR)

    WB analysis using 85983-1-RR (same clone as 85983-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85983-1-RR (PRKG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85983-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85983-1-RR

    Affinity and Kinetic Characterization of 85983-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85983-1-RR against Human PRKG1 were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

85983-1-PBS bindet in WB, Indirect ELISA PRKG1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen PRKG1 fusion protein Ag16333
Vollständiger Name protein kinase, cGMP-dependent, type I
Berechnetes Molekulargewicht 686 aa, 78 kDa
Beobachtetes Molekulargewicht 65-78 kDa
GenBank-ZugangsnummerBC127090
Gene symbol PRKG1
Gene ID (NCBI) 5592
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRKG1(cGMP-dependent protein kinase 1) is also named as cGK1, PRKG1B, PRKGR1A, PRKGR1B and belongs to the cGMP subfamily. It serves as an integral component of second messenger signaling in a number of biological contexts including cell differentiation, memory, and vasodilation (PMID:21893290). Its activity prevents pathological-level nitric oxide-induced apoptosis and promotes DNA synthesis/cell proliferation in vascular smooth muscle cells (PMID:20060325). PRKG1 has 2 isoforms produced by alternative splicing with the molecular weight of 76 kDa and 78 kDa. The autophosphorylation can increase the kinase activity and it can form a monomer with the molecular weight of 65 kDa that is produced by proteolytic cleavage. SDS-PAGE revealed that proteolysis generated two different monomers with molecular masses of 70 and 68 kDa of CGK1-beta(PMID:8702828).