PRKG2 Polyklonaler Antikörper

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• KD/KO Validated

PRKG2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

Kat-Nr. : 55138-1-AP

Synonyme

cGK2, cGKII, cGMP dependent protein kinase 2, cGMP-dependent protein kinase 2, cGMP-dependent protein kinase II

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  WB analysis of mouse brain using 55138-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55138-1-AP (PRKG2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 55138-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55138-1-AP (PRKG2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 55138-1-AP

  IP result of anti-PRKG2 (IP:55138-1-AP, 4ug; Detection:55138-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human colon using 55138-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 55138-1-AP (PRKG2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 55138-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 55138-1-AP (PRKG2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 55138-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 55138-1-AP (PRKG2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 55138-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 55138-1-AP (PRKG2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of C6 using 55138-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using PRKG2 antibody (55138-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of C6 using 55138-1-AP

  Immunofluorescent analysis of C6 cells using 55138-1-AP (PRKG2 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: C6-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

55138-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PRKG2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: protein kinase, cGMP-dependent, type II
• Berechnetes Molekulargewicht: 87 kDa
• Beobachtetes Molekulargewicht: 85-90 kDa
• GenBank-Zugangsnummer: NM_006259
• Gene symbol: PRKG2
• Gene ID (NCBI): 5593
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRKG2, also named as PRKGR2 and cGK2, belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and cGMP subfamily. It catalyses the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody is specific to PRKG2.

Publikationen

Species	Application	Title
human	WB,IHC	Oncogene; Transcriptional reprogramming triggered by neonatal UV radiation or Lkb1 loss prevents BRAFV600E-induced growth arrest in melanocytes; Authors - Kimberley McGrail
mouse	WB	EMBO Rep; Phosphorylation of myelin regulatory factor by PRKG2 mediates demyelination in Huntington's disease.; Authors - Peng Yin ; KO Validated
human	WB	Front Oncol; High-Risk HPV16 E6 Activates the cGMP/PKG Pathway Through Glycosyltransferase ST6GAL1 in Cervical Cancer Cells.; Authors - Jun Wang
human	WB	Mol Cell Endocrinol; EDNRB negatively regulates glycolysis to exhibit anti-tumor functions in prostate cancer by cGMP/PKG pathway; Authors - Xun Li
human	WB	Nutr Cancer; Erianin Exerts Antineoplastic Effects on Esophageal Squamous Cell Carcinoma Cells by Activating the cGMP-PKG Signaling Pathway; Authors - Xin Deng
human	WB	Cell Death Dis; The novel ZEB1-upregulated protein PRTG induced by Helicobacter pylori infection promotes gastric carcinogenesis through the cGMP/PKG signaling pathway.; Authors - Tian Xiang