PRKRA Monoklonaler Antikörper

PRKRA Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D4C3

Kat-Nr. : 68518-1-PBS

Synonyme

PACT, Interferon-inducible double-stranded RNA-dependent protein kinase activator A, HSD-14, HSD14, HSD 14

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  WB analysis using 68518-1-Ig (same clone as 68518-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68518-1-Ig (PRKRA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 68518-1-Ig (same clone as 68518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 68518-1-Ig (same clone as 68518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68518-1-Ig (same clone as 68518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68518-1-Ig (same clone as 68518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 68518-1-Ig (same clone as 68518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 68518-1-Ig (same clone as 68518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 68518-1-Ig (same clone as 68518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68518-1-PBS bindet in WB, IHC, Indirect ELISA PRKRA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PRKRA fusion protein Ag29818
• Vollständiger Name: protein kinase, interferon-inducible double stranded RNA dependent activator
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC009470
• Gene symbol: PRKRA
• Gene ID (NCBI): 8575
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRKRA (PKR-associated protein X) is also named as PACT, RAX, HSD-14 and HSD14. PRKRA was significantly overexpressed in PC tissues and promoted the proliferation, migration, and chemoresistance of PC cells (PMID: 37484321). PRKRA activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. PRKRA has been shown to interact with DICER1. DICER1 and PRKRA expression in normal colon was limited to mucosal epithelium and showed similar cytoplasmic expression (PMID: 37484321).