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PRKRIP1 Polyklonaler Antikörper

PRKRIP1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16794-1-PBS

Synonyme

C114, PRKR interacting protein 1, PRKR-interacting protein 1

Filter:
  • Western Blot (WB) analysis of various lysates using PRKRIP1 Polyclonal antibody (16794-1-AP)

    WB analysis using 16794-1-AP (same clone as 16794-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16794-1-AP (PRKRIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16794-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using PRKRIP1 Polyclonal antibody (16794-1-AP)

    IP experiment of NIH/3T3 using 16794-1-AP (same clone as 16794-1-PBS)

    IP result of anti-PRKRIP1 (IP:16794-1-AP, 4ug; Detection:16794-1-AP 1:2000) with NIH/3T3 cells lysate 1200 ug. This data was developed using the same antibody clone with 16794-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PRKRIP1 Polyclonal antibody (16794-1-AP)

    IHC staining of human colon cancer using 16794-1-AP (same clone as 16794-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16794-1-AP (PRKRIP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16794-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using PRKRIP1 Polyclonal antibody (16794-1-AP)

    IF Staining of Caco-2 using 16794-1-AP (same clone as 16794-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using PRKRIP1 antibody (16794-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16794-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

16794-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA PRKRIP1 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PRKRIP1 fusion protein Ag10266
Vollständiger Name PRKR interacting protein 1 (IL11 inducible)
Berechnetes Molekulargewicht 184 aa, 21 kDa
Beobachtetes Molekulargewicht21-25 kDa
GenBank-ZugangsnummerBC014298
Gene symbol PRKRIP1
Gene ID (NCBI) 79706
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRKR-interacting protein 1 (PRKRIP1), a component of the splicing C complex, is a double-stranded RNA-binding protein. PRKRIP1 consists of a fully extended N-terminal loop (residues 51-75) and an 18-turn α helix (residues 76-142) that spans 100 Å (PMID: 28502770). The full-length cDNA contains an open reading frame of 561 bp, which encodes an 184-amino acid polypeptide with a predicted molecular mass of 21 kDa (PMID: 12679338).