PRLR Monoklonaler Antikörper

PRLR Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2E10G5

Kat-Nr. : 67292-1-PBS

Synonyme

2E10G5, hPRLrI, PRL R, PRL-R, prolactin receptor

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  WB analysis using 67292-1-Ig (same clone as 67292-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67292-1-Ig (PRLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67292-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 67292-1-Ig (same clone as 67292-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 67292-1-Ig (PRLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67292-1-PBS in a different storage buffer formulation.

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  WB analysis of T-47D using 67292-1-Ig (same clone as 67292-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 67292-1-Ig (PRLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67292-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67292-1-Ig (same clone as 67292-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67292-1-Ig (PRLR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67292-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67292-1-Ig (same clone as 67292-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67292-1-Ig (PRLR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67292-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67292-1-PBS bindet in WB, IHC, Indirect ELISA PRLR und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PRLR fusion protein Ag28778
• Vollständiger Name: prolactin receptor
• Berechnetes Molekulargewicht: 622 aa, 70 kDa
• Beobachtetes Molekulargewicht: 45 kDa, 70 kDa, 85-95 kDa
• GenBank-Zugangsnummer: BC059392
• Gene symbol: PRLR
• Gene ID (NCBI): 5618
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRLR belongs to the cytokine type I subfamily, which also includes receptors for leptin, leukemia inhibiting factor, erythropoietin and so on. PRLR has long and short forms at approximately 70 and 45 kDa, respectively, and the mature, glycosylated form of PRLR migrates at 85-95 kDa in human decidua and placenta (PMID: 18258683).