PRM2 Polyklonaler Antikörper

PRM2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA, IF

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 14500-1-AP

Synonyme

Sperm histone HP2, Protamine-2, Basic nuclear protein HPS2, Basic nuclear protein HPS1, Basic nuclear protein HPI2

---

• 

  WB analysis of human testis using 14500-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14500-1-AP (PRM2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 14500-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14500-1-AP (PRM2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 14500-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14500-1-AP (PRM2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Produktinformation

14500-1-AP bindet in WB, IHC, ELISA, IF PRM2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRM2 fusion protein Ag5952
• Vollständiger Name: protamine 2
• Berechnetes Molekulargewicht: 13 kDa
• Beobachtetes Molekulargewicht: 10-25 kDa
• GenBank-Zugangsnummer: BC066338
• Gene symbol: PRM2
• Gene ID (NCBI): 5620
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRM2 (Protamine-2) is the main DNA-binding protein in the sperm nucleus, consisting of a C-terminal mature PRM2 (mPRM2) domain and an N-terminal lytic PRM2 (cPRM2) domain, which is lyzed sequentially after binding to DNA. PRM2 plays an important role in spermatogenesis and development. PRM2 is specifically expressed in sperm and localized in the sperm nucleus.

Publikationen

Species	Application	Title
human	WB	Mol Cell; Chromodomain Protein CDYL Acts as a Crotonyl-CoA Hydratase to Regulate Histone Crotonylation and Spermatogenesis.; Authors - Shumeng Liu
mouse	IF	J Hazard Mater; Multi-omics analyses reveal the mechanisms of Arsenic-induced male reproductive toxicity in mice.; Authors - Zijun Peng
mouse	WB	Environ Pollut; Silica nanoparticles induce spermatogenesis disorders via L3MBTL2-DNA damage-p53 apoptosis and RNF8-ubH2A/ubH2B pathway in mice.; Authors - Jianhui Liu
mouse	WB	Environ Pollut; Silica nanoparticles inhibiting the differentiation of round spermatid and chromatin remodeling of haploid period via MIWI in mice.; Authors - Jianhui Liu
human	WB	Hum Reprod; Lysine glutarylation in human sperm is associated with progressive motility.; Authors - Yi-Min Cheng
human		Hum Reprod; Posttranslational lysine 2-hydroxyisobutyrylation of human sperm tail proteins affects motility.; Authors - Yi-Min Cheng