PRMT1 Polyklonaler Antikörper

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• KD/KO Validated

PRMT1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(17)

Rezensionen (1)

Kat-Nr. : 11279-1-AP

Synonyme

ANM1, EC:2.1.1.319, HCP1, Histone-arginine N-methyltransferase PRMT1, HRMT1L2

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  WB analysis using 11279-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11279-1-AP (PRMT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 11279-1-AP

  WB result of PRMT1 antibody (11279-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRMT1 transfected A549 cells.

• 

  WB analysis using 11279-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11279-1-AP (PRMT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11279-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11279-1-AP (PRMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 11279-1-AP

  IP result of anti-PRMT1 (IP:11279-1-AP, 4ug; Detection:11279-1-AP 1:2000) with A549 cells lysate 920 ug.

• 

  IHC staining of human colon using 11279-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11279-1-AP (PRMT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 11279-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11279-1-AP (PRMT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 11279-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PRMT1 antibody (11279-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 11279-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PRMT1 Polyclonal antibody (11279-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, Jurkat-Zellen, MCF-7-Zellen, Maushirngewebe, NIH/3T3-Zellen, Rattenhirngewebe
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 14 publications below
• IHC: See 5 publications below
• IF: See 2 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

11279-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA PRMT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRMT1 fusion protein Ag1790
• Vollständiger Name: protein arginine methyltransferase 1
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 40-42 kDa
• GenBank-Zugangsnummer: BC019268
• Gene symbol: PRMT1
• Gene ID (NCBI): 3276
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRMT1, the major protein arginine methyltransferase in mammals, catalyzes monomethylation and asymmetric dimethylation of arginine side chains in proteins. Human PRMT1 is encoded by the PRMT1 gene located on chromosome 19 (19q13.3) and composed of 12 exons and 11 introns. At the protein level, human PRMT1 shares a high degree of homology with the different members of the PRMT family that is conserved in eukaryotes. Phylogenetic studies based on the methyltransferase domain highlighted that PRMT1 is closely related to PRMT8.

Publikationen

Species	Application	Title
mouse,human	WB,IP,IHC	Cell Metab; Thymidine kinase 1 drives hepatocellular carcinoma in enzyme-dependent and -independent manners; Authors - Qing Li
mouse	WB,IP	Nucleic Acids Res; RNA helicase D1PAS1 resolves R-loops and forms a complex for mouse pachytene piRNA biogenesis required for male fertility; Authors - Heejin Choi
human	WB	Cell Death Dis; PRMT1-mediated PGK1 arginine methylation promotes colorectal cancer glycolysis and tumorigenesis; Authors - Hao Liu
human	WB,IHC,CoIP	Cell Death Dis; TRIM21-mediated PRMT1 degradation attenuates colorectal cancer malignant progression; Authors - Menghan Cao
human	IHC	Oncogene; PRMT1 enhances oncogenic arginine methylation of NONO in colorectal cancer.; Authors - Xin-Ke Yin
mouse,human	WB,IHC	NPJ Precis Oncol; The SMARCA4R1157W mutation facilitates chromatin remodeling and confers PRMT1/SMARCA4 inhibitors sensitivity in colorectal cancer; Authors - Xiangwei Zeng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kyosuke (Verified Customer) (06-12-2019)

It works well on HUVECs for Western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HUVECs