PRMT1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PRMT1 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Rezensionen (1)

Kat-Nr. : 11279-1-AP

Synonyme

ANM1, HCP1, HMT2, HRMT1L2, IR1B4, PRMT1

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Galerie der Validierungsdaten

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  WB analysis of A549 using 11279-1-AP

  WB result of PRMT1 antibody (11279-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRMT1 transfected A549 cells.

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  WB analysis using 11279-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11279-1-AP (PRMT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11279-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11279-1-AP (PRMT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11279-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11279-1-AP (PRMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 11279-1-AP

  IP result of anti-PRMT1(IP:11279-1-AP, 4ug; Detection:11279-1-AP 1:2000) with A549 cells lysate 920 ug.

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  IHC staining of human colon using 11279-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11279-1-AP (PRMT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 11279-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11279-1-AP (PRMT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 11279-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PRMT1 antibody (11279-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of Raji using 11279-1-AP

  1X10^6 Raji cells were stained with 0.2ug PRMT1 antibody (11279-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, Jurkat-Zellen, Maushirngewebe, MCF-7-Zellen, NIH/3T3-Zellen, Rattenhirngewebe
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen
• Erfolgreiche Detektion in FC: Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Produktinformation

11279-1-AP bindet in WB, IP, IHC, IF, FC, ELISA PRMT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRMT1 fusion protein Ag1790
• Vollständiger Name: protein arginine methyltransferase 1
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 40-42 kDa
• GenBank-Zugangsnummer: BC019268
• Gene symbol: PRMT1
• Gene ID (NCBI): 3276
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRMT1, the major protein arginine methyltransferase in mammals, catalyzes monomethylation and asymmetric dimethylation of arginine side chains in proteins. Human PRMT1 is encoded by the PRMT1 gene located on chromosome 19 (19q13.3) and composed of 12 exons and 11 introns. At the protein level, human PRMT1 shares a high degree of homology with the different members of the PRMT family that is conserved in eukaryotes. Phylogenetic studies based on the methyltransferase domain highlighted that PRMT1 is closely related to PRMT8.

Publikationen

Species	Application	Title
human,mouse	WB,IHC,IP	Cell Metab; Thymidine kinase 1 drives hepatocellular carcinoma in enzyme-dependent and -independent manners; Authors - Qing Li
human	IHC	Oncogene; PRMT1 enhances oncogenic arginine methylation of NONO in colorectal cancer.; Authors - Xin-Ke Yin
human,mouse	WB,IHC	NPJ Precis Oncol; The SMARCA4R1157W mutation facilitates chromatin remodeling and confers PRMT1/SMARCA4 inhibitors sensitivity in colorectal cancer; Authors - Xiangwei Zeng
mouse	WB	Diabetes; PRMT4 Facilitates White Adipose Tissue Browning and Thermogenesis by Methylating PPARγ; Authors - Yi Zhong
mouse	WB,IF	Front Mol Neurosci; Cingulate protein arginine methyltransferases 1 regulates peripheral hypersensitivity via fragile X messenger ribonucleoprotein; Authors - Cheng Wu ; KD Validated
human	WB	Toxicol Appl Pharmacol; Ribavirin inhibits the growth and ascites formation of hepatocellular carcinoma through downregulation of type I CARM1 and type II PRMT5; Authors - Yizhen Tian

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kyosuke (Verified Customer) (06-12-2019)

It works well on HUVECs for Western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HUVECs