PRMT2 Monoklonaler Antikörper

PRMT2 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

CloneNo.

1F1B9

Kat-Nr. : 66885-1-Ig

Synonyme

HMT1, HRMT1L1, PRMT2

---

• 

  WB analysis of SKOV-3 using 66885-1-Ig

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 66885-1-Ig (PRMT2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 66885-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66885-1-Ig (PRMT2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66885-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66885-1-Ig (PRMT2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 66885-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66885-1-Ig (PRMT2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 4T1 using 66885-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 66885-1-Ig (PRMT2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66885-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66885-1-Ig (PRMT2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human thyroid cancer using 66885-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66885-1-Ig (PRMT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 66885-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66885-1-Ig (PRMT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66885-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PRMT2 antibody (66885-1-Ig, Clone: 1F1B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SKOV-3-Zellen, 4T1-Zellen, HeLa-Zellen, Jurkat-Zellen, LNCaP-Zellen, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Schilddrüsenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

66885-1-Ig bindet in WB, IHC, IF/ICC, ELISA PRMT2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PRMT2 fusion protein Ag1806
• Vollständiger Name: protein arginine methyltransferase 2
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC000727
• Gene symbol: PRMT2
• Gene ID (NCBI): 3275
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRMT2 (also known as HRMT1L1) is a member of the arginine methyltransferase family which catalyzes arginine methylation and regulates diverse cellular processes, including transcription, translation, DNA repair, and RNA processing. Recently PRMT2 has been identified as a pro-tumorigenic factor in malignant gliomas.

Publikationen

Species	Application	Title
human	WB	ACS Omega; Biochemical Properties of CARM1: Impact on Western Blotting and Proteomic Studies; Authors - Julie Bourassa
human	WB	Toxicol Appl Pharmacol; Ribavirin inhibits the growth and ascites formation of hepatocellular carcinoma through downregulation of type I CARM1 and type II PRMT5; Authors - Yizhen Tian
human,mouse	WB	Basic Clin Pharmacol Toxicol; Ribavirin inhibits cell proliferation and metastasis and prolongs survival in soft tissue sarcomas by downregulating both protein arginine methyltransferases 1 and 5.; Authors - Qingqing Zhang
human	WB,IHC	Cell Death Dis; PRMT2 promotes RCC tumorigenesis and metastasis via enhancing WNT5A transcriptional expression; Authors - Zhongwei Li
rat	WB,IF	Neurochem Int; The antidepressant effects of protein arginine methyltransferase 2 involve neuroinflammation; Authors - Shunfeng Liu
human	WB	Int J Mol Sci; Human C15orf39 Inhibits Inflammatory Response via PRMT2 in Human Microglial HMC3 Cell Line; Authors - Min Zhang