PRMT2 Monoklonaler Antikörper

PRMT2 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F1B9

Kat-Nr. : 66885-1-PBS

Synonyme

HMT1, HRMT1L1, PRMT2

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  WB analysis of SKOV-3 using 66885-1-Ig (same clone as 66885-1-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 66885-1-Ig (PRMT2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66885-1-PBS in a different storage buffer formulation.

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  WB analysis of rat heart using 66885-1-Ig (same clone as 66885-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66885-1-Ig (PRMT2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66885-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 66885-1-Ig (same clone as 66885-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66885-1-Ig (PRMT2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66885-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66885-1-Ig (same clone as 66885-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66885-1-Ig (PRMT2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66885-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 66885-1-Ig (same clone as 66885-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 66885-1-Ig (PRMT2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66885-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66885-1-Ig (same clone as 66885-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66885-1-Ig (PRMT2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66885-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 66885-1-Ig (same clone as 66885-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66885-1-Ig (PRMT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66885-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 66885-1-Ig (same clone as 66885-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66885-1-Ig (PRMT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66885-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66885-1-Ig (same clone as 66885-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PRMT2 antibody (66885-1-Ig, Clone: 1F1B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66885-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66885-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PRMT2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PRMT2 fusion protein Ag1806
• Vollständiger Name: protein arginine methyltransferase 2
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC000727
• Gene symbol: PRMT2
• Gene ID (NCBI): 3275
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRMT2 (also known as HRMT1L1) is a member of the arginine methyltransferase family which catalyzes arginine methylation and regulates diverse cellular processes, including transcription, translation, DNA repair, and RNA processing. Recently PRMT2 has been identified as a pro-tumorigenic factor in malignant gliomas.