PRMT3 Rekombinanter Antikörper

PRMT3 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

241092E6

Kat-Nr. : 83969-7-PBS

Synonyme

Protein arginine N-methyltransferase 3, PRMT 3, HRMT1L3, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3, EC:2.1.1.319

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  WB analysis using 83969-7-RR (same clone as 83969-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83969-7-RR (PRMT3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83969-7-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83969-7-RR (same clone as 83969-7-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRMT3 antibody (83969-7-RR, Clone: 241092E6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83969-7-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 83969-7-RR (same clone as 83969-7-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug PRMT3 Recombinant antibody (83969-7-RR, Clone:241092E6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83969-7-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

83969-7-PBS bindet in WB, IF/ICC, FC (Intra), ELISA PRMT3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PRMT3 fusion protein Ag13466
• Vollständiger Name: protein arginine methyltransferase 3
• Berechnetes Molekulargewicht: 531 aa, 60 kDa
• Beobachtetes Molekulargewicht: 60-68 kDa
• GenBank-Zugangsnummer: BC037544
• Gene symbol: PRMT3
• Gene ID (NCBI): 10196
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRMT3(protein arginine methyltransferase 3) regulates protein functions by introducing asymmetric dimethylation marks at the arginine residues in proteins.