PRMT5 Polyklonaler Antikörper

PRMT5 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27109-1-AP

Synonyme

72 kDa ICln-binding protein, EC:2.1.1.320, Histone-arginine N-methyltransferase PRMT5, Jak-binding protein 1, Protein arginine N methyltransferase 5

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  WB analysis using 27109-1-AP

  Various cells were subjected to SDS PAGE followed by western blot with 27109-1-AP (PRMT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27109-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27109-1-AP (PRMT5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 27109-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 27109-1-AP (PRMT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 27109-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27109-1-AP (PRMT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 27109-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 27109-1-AP (PRMT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, HL-60-Zellen, NIH/3T3-Zellen, PC-12-Zellen, Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

27109-1-AP bindet in WB, ELISA PRMT5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRMT5 fusion protein Ag25896
• Vollständiger Name: protein arginine methyltransferase 5
• Berechnetes Molekulargewicht: 637 aa, 73 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC025979
• Gene symbol: PRMT5
• Gene ID (NCBI): 10419
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRMT5 is a Type II enzyme that catalyzes arginine monomethylation and symmetric dimethylation (Rme1 and Rme2s). The many targets of PRMT5 include ribosomal proteins, the histone chaperone nucleoplasmin, p53, and histones. PRMT5 is frequently observed in a complex with the cofactor, methylosome protein 50 (MEP50), which is required for PRMT5 activity. PRMT5 is upregulated in several human malignancies, including lymphomas, lung cancer, breast cancer and colorectal cancer.