PRMT6 Polyklonaler Antikörper

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• KD/KO Validated

PRMT6 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 15395-1-AP

Synonyme

HRMT1L6, PRMT6

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Galerie der Validierungsdaten

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  WB analysis using 15395-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15395-1-AP (PRMT6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15395-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15395-1-AP (PRMT6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 15395-1-AP

  IP Result of anti-PRMT6 (IP:15395-1-AP, 4ug; Detection:15395-1-AP 1:1500) with HEK-293 cells lysate 2400ug.

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  IHC staining of human prostate cancer using 15395-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15395-1-AP (PRMT6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 15395-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15395-1-AP (PRMT6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 15395-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15395-1-AP (PRMT6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 15395-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15395-1-AP (PRMT6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human breast cancer using 15395-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15395-1-AP (PRMT6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15395-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15395-1-AP (PRMT6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15395-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15395-1-AP (PRMT6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15395-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15395-1-AP (PRMT6 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of MCF-7 using 15395-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 15395-1-AP (PRMT6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Maushirngewebe, MCF-7-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Hodengewebe, humanes Mammakarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

15395-1-AP bindet in WB, IP, IHC, IF, ELISA PRMT6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRMT6 fusion protein Ag7592
• Vollständiger Name: protein arginine methyltransferase 6
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC002729
• Gene symbol: PRMT6
• Gene ID (NCBI): 55170
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRMT6 belongs to the protein arginine methyltransferase (PRMT) family that catalyzes the mono- and dimethylation of arginine residues in a variety of proteins. PRMT6 is a nuclear protein and is predominantly expressed in testis and kidney. Western blot analysis using this antibody detected a major band around 42 kDa in HeLa cells.

Publikationen

Species	Application	Title
human,mouse	WB	EMBO Rep; Arginine methylation of SIRT7 couples glucose sensing with mitochondria biogenesis.; Authors - Wei-Wei Yan ; KD Validated
human	WB	PLoS Pathog; SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.; Authors - Muyu Xu
mouse	WB	Mol Metab; Disrupted Liver Oxidative Metabolism in Glycine N-Methyltransferase-Deficient Mice is Mitigated by Dietary Methionine Restriction.; Authors - Ferrol I Rome
mouse	IHC	Aging (Albany NY); Cigarette smoke extract induces airway epithelial cell death via repressing PRMT6/AKT signaling.; Authors - Tiao Li
human	WB	Front Cell Dev Biol; F-Box Protein FBXW17-Mediated Proteasomal Degradation of Protein Methyltransferase PRMT6 Exaggerates CSE-Induced Lung Epithelial Inflammation and Apoptosis.; Authors - Tiao Li
human	WB	Biochem Pharmacol; PMID: 32535103; Authors - Qi Feng ; KD Validated