PRMT6 Monoklonaler Antikörper

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• KD/KO Validated

PRMT6 Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4B5A9

Kat-Nr. : 67981-1-PBS

Synonyme

4B5A9, EC:2.1.1.319, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6, Histone-arginine N-methyltransferase PRMT6, HRMT1L6

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  WB analysis using 67981-1-Ig (same clone as 67981-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67981-1-Ig (PRMT6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67981-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 67981-1-Ig (same clone as 67981-1-PBS)

  WB result of PRMT6 antibody (67981-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRMT6 transfected MCF-7 cells. This data was developed using the same antibody clone with 67981-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67981-1-PBS bindet in WB, Indirect ELISA PRMT6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PRMT6 fusion protein Ag7934
• Vollständiger Name: protein arginine methyltransferase 6
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC002729
• Gene symbol: PRMT6
• Gene ID (NCBI): 55170
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein arginine methyltransferase 6 (PRMT6) is a type I PRMT which is involved in epigenetic regulation of gene expression, alternative splicing, development and differentiation, DNA repair, cell proliferation and senescence, DNA methylation, mitosis, inflammation, innate antiviral immunity, spermatogenesis, transactivation of nuclear receptors and cell signaling. The human PRMT6 gene, located on Chromosome 1, encodes for the 41.9 kDa PRMT6 enzyme. PRMT6 is predominantly localized to the nucleus, in stark contrast to PRMT3 and PRMT5 which are preponderantly cytosolic, while other PRMTs are found in both nucleus and cytosol. PRMT6 is expressed in a wide range of tissues with high expression in kidney and testes. PRMT6 generates asymmetric dimethylation modifications in histone 3 at arginine 2, arginine 17 and arginine 42 (H3R2me2a, H3R17me2a and H3R42me2a) and in histone H2A at arginine 26 (H2AR26me2a) and participates in the epigenetic regulation of gene expression.