PRMT7 Polyklonaler Antikörper

PRMT7 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21688-1-AP

Synonyme

Protein arginine N-methyltransferase 7, PRMT 7, Histone-arginine N-methyltransferase PRMT7, EC:2.1.1.321, [Myelin basic protein]-arginine N-methyltransferase PRMT7

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  WB analysis using 21688-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21688-1-AP (PRMT7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21688-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21688-1-AP (PRMT7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 21688-1-AP

  IP result of anti-PRMT7 (IP:21688-1-AP, 4ug; Detection:21688-1-AP 1:1000) with HeLa cells lysate 1400 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, HeLa-Zellen, MCF-7-Zellen, MDA-MB-231-Zellen
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

21688-1-AP bindet in WB, IP, ELISA PRMT7 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRMT7 fusion protein Ag16269
• Vollständiger Name: protein arginine methyltransferase 7
• Berechnetes Molekulargewicht: 78 kDa
• Beobachtetes Molekulargewicht: 73-78 kDa
• GenBank-Zugangsnummer: BC000146
• Gene symbol: PRMT7
• Gene ID (NCBI): 54496
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein arginine methyltransferases (PRMTs) are responsible for arginine methylation of histone or nonhistone proteins to regulate diverse biological processes, including cell proliferation, differentiation and senescence. PRMT7 is expressed in both embryonic stem and germ cells and is implicated in maintenance of muscle stem cell function. overexpression of PRMT7 has been associated with cancer metastasis. PRMT7 automethylation at residue R531 was reported to enhance breast cancer metastasis.