PRMT7 Monoklonaler Antikörper

PRMT7 Monoklonal Antikörper für WB,ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

3C5H6

Kat-Nr. : 67669-1-Ig

Synonyme

FLJ10640, KIAA1933, PRMT7

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Galerie der Validierungsdaten

• 

  WB analysis using 67669-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67669-1-Ig (PRMT7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67669-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67669-1-Ig (PRMT7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67669-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67669-1-Ig (PRMT7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

67669-1-Ig bindet in WB, ELISA PRMT7 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PRMT7 fusion protein Ag30434
• Vollständiger Name: protein arginine methyltransferase 7
• Berechnetes Molekulargewicht: 78 kDa
• Beobachtetes Molekulargewicht: 78 kDa
• GenBank-Zugangsnummer: BC000146
• Gene symbol: PRMT7
• Gene ID (NCBI): 54496
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein arginine methyltransferases (PRMTs) are responsible for arginine methylation of histone or nonhistone proteins to regulate diverse biological processes, including cell proliferation, differentiation and senescence. PRMT7 is expressed in both embryonic stem and germ cells and is implicated in maintenance of muscle stem cell function. overexpression of PRMT7 has been associated with cancer metastasis. PRMT7 automethylation at residue R531 was reported to enhance breast cancer metastasis.

Publikationen

Species	Application	Title
human	WB	Gastroenterology; Genetic Control of Alternative Splicing and its Distinct Role in Colorectal Cancer Mechanisms; Authors - Ming Zhang
human	WB	EMBO J; MST2 methylation by PRMT5 inhibits Hippo signaling and promotes pancreatic cancer progression; Authors - Yan Sun