PRMT7 Monoklonaler Antikörper

PRMT7 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3C5H6

Kat-Nr. : 67669-1-PBS

Synonyme

FLJ10640, KIAA1933, PRMT7

---

• 

  WB analysis using 67669-1-Ig (same clone as 67669-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67669-1-Ig (PRMT7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67669-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 67669-1-Ig (same clone as 67669-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67669-1-Ig (PRMT7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67669-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67669-1-Ig (same clone as 67669-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67669-1-Ig (PRMT7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67669-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Jurkat using 67669-1-Ig (same clone as 67669-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PRMT7 antibody (67669-1-Ig, Clone: 3C5H6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67669-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

67669-1-PBS bindet in WB, IF/ICC, Indirect ELISA PRMT7 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PRMT7 fusion protein Ag30434
• Vollständiger Name: protein arginine methyltransferase 7
• Berechnetes Molekulargewicht: 78 kDa
• Beobachtetes Molekulargewicht: 78 kDa
• GenBank-Zugangsnummer: BC000146
• Gene symbol: PRMT7
• Gene ID (NCBI): 54496
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein arginine methyltransferases (PRMTs) are responsible for arginine methylation of histone or nonhistone proteins to regulate diverse biological processes, including cell proliferation, differentiation and senescence. PRMT7 is expressed in both embryonic stem and germ cells and is implicated in maintenance of muscle stem cell function. overexpression of PRMT7 has been associated with cancer metastasis. PRMT7 automethylation at residue R531 was reported to enhance breast cancer metastasis.