PRMT8 Polyklonaler Antikörper

PRMT8 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 12557-1-AP

Synonyme

HRMT1L3, HRMT1L4, PRMT8

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  WB analysis of Y79 using 12557-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12557-1-AP (PRMT8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 12557-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12557-1-AP (PRMT8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12557-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12557-1-AP (PRMT8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12557-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12557-1-AP (PRMT8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12557-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12557-1-AP (PRMT8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Y79-Zellen
• Erfolgreiche Detektion in IHC: Rattenhirngewebe, humanes Mammakarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

12557-1-AP bindet in WB, IHC, ELISA PRMT8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRMT8 fusion protein Ag3166
• Vollständiger Name: protein arginine methyltransferase 8
• Berechnetes Molekulargewicht: 394 aa, 45 kDa
• Beobachtetes Molekulargewicht: 43-50 kDa
• GenBank-Zugangsnummer: BC022458
• Gene symbol: PRMT8
• Gene ID (NCBI): 56341
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRMT8 is a member of arginine methyl transferase (PRMT) family which acts as nuclear receptor coactivators. PRMT8 catalyzes the asymmetric arginine methylation and is involved in cellular differentiation. PRMT8 has several isoforms; the full-length isoform has a brain specific expression pattern and is localized to the plasma membrane; the truncated isoforms display nuclear localization and are expressed more widely. This antibody recognizes all of isoforms of PRMT8.

Publikationen

Species	Application	Title
human	WB	Toxicol Appl Pharmacol; Ribavirin inhibits the growth and ascites formation of hepatocellular carcinoma through downregulation of type I CARM1 and type II PRMT5; Authors - Yizhen Tian
human,mouse	WB	Basic Clin Pharmacol Toxicol; Ribavirin inhibits cell proliferation and metastasis and prolongs survival in soft tissue sarcomas by downregulating both protein arginine methyltransferases 1 and 5.; Authors - Qingqing Zhang