PRND Polyklonaler Antikörper

PRND Polyklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26947-1-PBS

Synonyme

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  WB analysis using 26947-1-AP (same clone as 26947-1-PBS)

  Non-treated mouse testis and glucosidase treated mouse testis tissue were subjected to SDS PAGE followed by western blot with 26947-1-AP (PRND antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26947-1-PBS in a different storage buffer formulation.

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  WB analysis using 26947-1-AP (same clone as 26947-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26947-1-AP (PRND antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26947-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 26947-1-AP (same clone as 26947-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26947-1-AP (PRND antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26947-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 26947-1-AP (same clone as 26947-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26947-1-AP (PRND antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26947-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 26947-1-AP (same clone as 26947-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PRND antibody (26947-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26947-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 26947-1-AP (same clone as 26947-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PRND antibody (26947-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26947-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

26947-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA PRND und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRND fusion protein Ag25523
• Vollständiger Name: prion protein 2 (dublet)
• Berechnetes Molekulargewicht: 176 aa, 20 kDa
• Beobachtetes Molekulargewicht: 17-20, 30 kDa
• GenBank-Zugangsnummer: BC043644
• Gene symbol: PRND
• Gene ID (NCBI): 23627
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The prion gene family is mainly composed of three genes; PRNP, encoding the prion protein (PrP), PRND, encoding Doppel (Dpl), both located on the same genomic locus on mouse chromosome 2 and SPRN, encoding Shadoo (Sho), located on mouse chromosome 7. PRND (prion protein 2 also called Doppel) is a membranean N-glycosylated, glycosylphosphatidylinositol-anchored protein that is found predominantly in testis. PRND gene is found on chromosome 20, approximately 20 kbp downstream of the gene encoding cellular prionprotein, to which it is biochemically and structurally similar. 26947-1-AP antibody detects a 30 kDa glycosylated protein and a 17-20 kDa protein treated by glucosidase.