EPCR/CD201 Polyklonaler Antikörper

EPCR/CD201 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32134-1-AP

Synonyme

Activated protein C receptor, APC receptor, CD201, Centrocyclin, Centrosomal protein CCD41

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  WB analysis using 32134-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 32134-1-AP (EPCR/CD201 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 32134-1-AP

  bEnd.3 cells were subjected to SDS PAGE followed by western blot with 32134-1-AP (EPCR/CD201 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 32134-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 32134-1-AP (EPCR/CD201 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 32134-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 32134-1-AP (EPCR/CD201 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen, bEnd.3-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

32134-1-AP bindet in WB, IHC, ELISA EPCR/CD201 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: protein C receptor, endothelial
• Berechnetes Molekulargewicht: 27kDa
• Beobachtetes Molekulargewicht: 27 kDa, 40-50 kDa
• GenBank-Zugangsnummer: NM_011171.2
• Gene symbol: PROCR
• Gene ID (NCBI): 19124
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein C receptor (PROCR), also known as activated protein C receptor (APC receptor), EPCR, or CD201, is a transmembrane glycoprotein. EPCR promotes the activation of protein C, which has anticoagulant and cytoprotective effects. EPCR facilitates the activation of protein C by the thrombin-thrombomodulin complex but also facilitates APC-mediated cytoprotective effects on cells that involve the activation of protease-activated receptors (PAR) 1 and 3(PMID: 27207424). EPCR is approximately 46 kDa, truncated EPCR is 23 kDa, truncated EPCR with the transmembrane domain is 26 kDa, and soluble EPCR is 43 kDa (PMID: 11686350).