EPCR/CD201 Monoklonaler Antikörper

EPCR/CD201 Monoklonal Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

1F5C4

Kat-Nr. : 67658-1-PBS

Synonyme

CD201, EPCR, PROCR, 1F5C4, Activated protein C receptor

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  WB analysis using 67658-1-Ig (same clone as 67658-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67658-1-Ig (PROCR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67658-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67658-1-Ig (same clone as 67658-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67658-1-Ig (PROCR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67658-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 67658-1-Ig (same clone as 67658-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67658-1-Ig (PROCR antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67658-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 67658-1-Ig (same clone as 67658-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67658-1-Ig (PROCR antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67658-1-PBS in a different storage buffer formulation.

• 

  FC experiment of A549 using 67658-1-Ig (same clone as 67658-1-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.4 ug PROCR Monoclonal antibody (67658-1-Ig, Clone:1F5C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67658-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67658-1-PBS bindet in WB, IHC, FC (Intra), ELISA EPCR/CD201 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EPCR/CD201 fusion protein Ag25628
• Vollständiger Name: protein C receptor, endothelial (EPCR)
• Berechnetes Molekulargewicht: 238 aa, 27 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC014451
• Gene symbol: PROCR
• Gene ID (NCBI): 10544
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.