EPCR/CD201 Rekombinanter Antikörper

EPCR/CD201 Rekombinant Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

241853A9

Kat-Nr. : 84494-2-RR

Synonyme

241853A9, Activated protein C receptor, APC receptor, CD201, Centrocyclin

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  WB analysis using 84494-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84494-2-RR (EPCR/CD201 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse placenta using 84494-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 84494-2-RR (EPCR/CD201 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse placenta using 84494-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 84494-2-RR (EPCR/CD201 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse placenta using 84494-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse placenta tissue using EPCR/CD201 antibody (84494-2-RR, Clone: 241853A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of bEnd.3 using 84494-2-RR

  Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using EPCR/CD201 antibody (84494-2-RR, Clone: 241853A9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, Maus-Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: Maus-Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Plazenta-Gewebe
• Erfolgreiche Detektion in IF/ICC: bEnd.3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84494-2-RR bindet in WB, IHC, IF/ICC, IF-P, ELISA EPCR/CD201 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: EPCR/CD201 fusion protein Eg1517
• Vollständiger Name: protein C receptor, endothelial
• Berechnetes Molekulargewicht: 27kDa
• Beobachtetes Molekulargewicht: 40-50 kDa
• GenBank-Zugangsnummer: NM_011171.2
• Gene symbol: PROCR
• Gene ID (NCBI): 19124
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein C receptor (PROCR), also known as activated protein C receptor (APC receptor), EPCR, or CD201, is a transmembrane glycoprotein. EPCR promotes the activation of protein C, which has anticoagulant and cytoprotective effects. EPCR facilitates the activation of protein C by the thrombin-thrombomodulin complex but also facilitates APC-mediated cytoprotective impact on cells that involve the activation of protease-activated receptors (PAR) 1 and 3(PMID: 27207424).