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CD133-1,2 Polyklonaler Antikörper

CD133-1,2 Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19946-1-AP

Synonyme

AC133, Antigen AC133, CD133, CORD12, MCDR2, MSTP061, PROM1, PROM1 1, PROM1 1,2, PROM1 1,2,3,5,7, PROM1 1,6,7, PROM1 2, PROM1 2,3,4,5, prominin 1, Prominin like protein 1, PROML1, RP41, STGD4

Galerie der Validierungsdaten

Filter:
  • Western Blot (WB) analysis of HT-29 cells using CD133-1,2 Polyclonal antibody (19946-1-AP)

    WB analysis of HT-29 using 19946-1-AP

    HT-29 cells were subjected to SDS PAGE followed by western blot with 19946-1-AP (CD133-1,2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Caco-2 cells using CD133-1,2 Polyclonal antibody (19946-1-AP)

    WB analysis of Caco-2 using 19946-1-AP

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 19946-1-AP (CD133-1,2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Y79 cells using CD133-1,2 Polyclonal antibody (19946-1-AP)

    IP experiment of Y79 using 19946-1-AP

    IP Result of anti-CD133-1,2,3 (IP:19946-1-AP, 4ug; Detection:19946-1-AP 1:300) with Y79 cells lysate 2000ug.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHT-29-Zellen, Caco-2-Zellen
Erfolgreiche IPY79-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

19946-1-AP bindet in WB, IP,ELISA CD133-1,2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name prominin 1
Berechnetes Molekulargewicht 97 kDa
Beobachtetes Molekulargewicht 110-120 kDa
GenBank-ZugangsnummerNM_006017
Gene symbol PROM1
Gene ID (NCBI) 8842
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD133, also known as PROM1 (prominin-1) or AC133, belongs to the prominin family. CD133 is a transmembrane glycoprotein with an NH2-terminal extracellular domain, five transmembrane loops and a cytoplasmic tail. The expression of CD133 has been reported in hematopoietic stem cells, endothelial progenitor cells, neuronal and glial stem cells, suggesting the potential role of CD133 as a cell surface marker of adult stem cells. CD133 has also been reported as a marker of cancer stem cells in various human tumors. This antibody recognizes transcript variant 1,2 of CD133.

Protokolle

Produktspezifische Protokolle
WB protocol for CD133-1,2 antibody 19946-1-APProtokoll herunterladen
IP protocol for CD133-1,2 antibody 19946-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen