CD133 Polyklonaler Antikörper

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• KD/KO Validated

CD133 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(262)

Kat-Nr. : 18470-1-AP

Synonyme

PROM1, MSTP061, MCDR2, CORD12, Antigen AC133

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  WB analysis using 18470-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18470-1-AP (CD133 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 18470-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18470-1-AP (PROM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 18470-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 18470-1-AP (PROM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-29 using 18470-1-AP

  HT-29 cells were subjected to SDS PAGE followed by western blot with 18470-1-AP (CD133 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18470-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18470-1-AP (CD133 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 18470-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18470-1-AP (CD133 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 18470-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18470-1-AP (CD133 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 18470-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CD133 antibody (18470-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, Caco-2-Zellen, Mausnierengewebe, Maus-Pankreasgewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausnierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:4000-1:16000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 177 publications below
• IHC: See 58 publications below
• IF: See 56 publications below
• FC: See 19 publications below

Produktinformation

18470-1-AP bindet in WB, IHC, IF-P, ELISA CD133 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD133 fusion protein Ag13328
• Vollständiger Name: prominin 1
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 115 kDa-120 kDa
• GenBank-Zugangsnummer: BC012089
• Gene symbol: CD133
• Gene ID (NCBI): 8842
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD133, also known as PROM1 (prominin-1) or AC133, belongs to the prominin family. CD133 is a transmembrane glycoprotein with an NH2-terminal extracellular domain, five transmembrane loops and a cytoplasmic tail. The expression of CD133 has been reported in hematopoietic stem cells, endothelial progenitor cells, neuronal and glial stem cells, suggesting the potential role of CD133 as a cell surface marker of adult stem cells. CD133 has also been reported as a marker of cancer stem cells in various human tumors.

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; Merkel cell polyomavirus activates LSD1-mediated blockade of non-canonical BAF to regulate transformation and tumorigenesis.; Authors - Donglim Esther Park
human	WB	Adv Sci (Weinh); Exosomal CCT6A Secreted by Cancer-Associated Fibroblasts Interacts with β-Catenin to Enhance Chemoresistance and Tumorigenesis in Gastric Cancer; Authors - Hui Sun
human	WB	Cell Rep Med; CD97 maintains tumorigenicity of glioblastoma stem cells via mTORC2 signaling and is targeted by CAR Th9 cells; Authors - Shuchang Zhou
human	WB	Cell Death Differ; CRSP8 promotes thyroid cancer progression by antagonizing IKKα-induced cell differentiation.; Authors - Yina Liao
human	WB	J Pineal Res; Melatonin inhibits the stemness of head and neck squamous cell carcinoma by modulating HA synthesis via the FOSL1/HAS3 axis; Authors - Xinyue Luo
human	WB,IF	Neuro Oncol; TRIM24 Promotes Stemness and Invasiveness of Glioblastoma Cells via Activating SOX2 Expression.; Authors - Lu-Hua Zhang