CD133 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CD133 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

216

Kat-Nr. : 18470-1-AP

Synonyme

AC133, Antigen AC133, CD133, CORD12, MCDR2, MSTP061, PROM1, PROM1 1, PROM1 1,2,3, PROM1 1,2,3,5,7, PROM1 1,6,7, PROM1 2, PROM1 2,3,4,5, prominin 1, Prominin like protein 1, PROML1, RP41, STGD4

---

Galerie der Validierungsdaten

• 

  WB analysis using 18470-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18470-1-AP (CD133 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 18470-1-AP

  HT-29 cells were subjected to SDS PAGE followed by western blot with 18470-1-AP (CD133 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18470-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18470-1-AP (CD133 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 18470-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18470-1-AP (PROM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 18470-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 18470-1-AP (PROM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 18470-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18470-1-AP (CD133 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 18470-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18470-1-AP (CD133 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 18470-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CD133 antibody (18470-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HT-29 using 18470-1-AP

  1X10^6 HT-29 cells were intracellularly stained with 0.4 ug Anti-Human CD133 (18470-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, Caco-2-Zellen, Mausnierengewebe, Maus-Pankreasgewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mausnierengewebe
• Erfolgreiche Detektion in FC: HT-29-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:4000-1:16000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 143 publications below
• IHC: See 48 publications below
• IF: See 49 publications below
• FC: See 18 publications below

Produktinformation

18470-1-AP bindet in WB, IHC, IF, FC, ELISA CD133 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD133 fusion protein Ag13328
• Vollständiger Name: prominin 1
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 115 kDa-120 kDa
• GenBank-Zugangsnummer: BC012089
• Gene symbol: PROM1
• Gene ID (NCBI): 8842
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD133, also known as PROM1 (prominin-1) or AC133, belongs to the prominin family. CD133 is a transmembrane glycoprotein with an NH2-terminal extracellular domain, five transmembrane loops and a cytoplasmic tail. The expression of CD133 has been reported in hematopoietic stem cells, endothelial progenitor cells, neuronal and glial stem cells, suggesting the potential role of CD133 as a cell surface marker of adult stem cells. CD133 has also been reported as a marker of cancer stem cells in various human tumors.

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; Merkel cell polyomavirus activates LSD1-mediated blockade of non-canonical BAF to regulate transformation and tumorigenesis.; Authors - Donglim Esther Park
human	WB	Cell Death Differ; CRSP8 promotes thyroid cancer progression by antagonizing IKKα-induced cell differentiation.; Authors - Yina Liao
human	WB,IF	Neuro Oncol; TRIM24 Promotes Stemness and Invasiveness of Glioblastoma Cells via Activating SOX2 Expression.; Authors - Lu-Hua Zhang
human,mouse	WB,IHC	PLoS Biol; A novel TOX3-WDR5-ABCG2 signaling axis regulates the progression of colorectal cancer by accelerating stem-like traits and chemoresistance; Authors - Jiaojiao Hao
human	WB	Theranostics; Carcinoma-associated fibroblasts promote the stemness and chemoresistance of colorectal cancer by transferring exosomal lncRNA H19.; Authors - Jing Ren
human	WB,IHC	Cancer Res; STRAP promotes stemness of human colorectal cancer via epigenetic regulation of the NOTCH pathway.; Authors - Lin Jin

Verwandte Produkte

CD34 Polyclonal antibody

14486-1-AP

CD44 Monoclonal antibody

60224-1-Ig

CD44 Polyclonal antibody

15675-1-AP

CXCR4 Polyclonal antibody

11073-2-AP

CXCR4 Monoclonal antibody

60042-1-Ig

ALDH1A1 Polyclonal antibody

15910-1-AP

EGFR-Specific Polyclonal antibody

18986-1-AP