CD133 Polyklonaler Antikörper

CD133 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 18495-1-AP

Synonyme

PROM1, MSTP061, MCDR2, CORD12, Antigen AC133

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  WB analysis using 18495-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18495-1-AP (CD133 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 18495-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18495-1-AP (PROM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 18495-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 18495-1-AP (CD133 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 18495-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 18495-1-AP (CD133 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 18495-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 18495-1-AP (CD133 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 18495-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 18495-1-AP (CD133 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, HT-29-Zellen, Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: human intrahepatic cholangiocarcinoma tissue, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IF: See 2 publications below

Produktinformation

18495-1-AP bindet in WB, IHC, IF, ELISA CD133 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD133 fusion protein Ag13327
• Vollständiger Name: prominin 1
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 110-115 kDa
• GenBank-Zugangsnummer: BC012089
• Gene symbol: CD133
• Gene ID (NCBI): 8842
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD133, also known as PROM1 (prominin-1) or AC133, belongs to the prominin family. CD133 is a transmembrane glycoprotein with an NH2-terminal extracellular domain, five transmembrane loops and a cytoplasmic tail. The expression of CD133 has been reported in hematopoietic stem cells, endothelial progenitor cells, neuronal and glial stem cells, suggesting the potential role of CD133 as a cell surface marker of adult stem cells. CD133 has also been reported as a marker of cancer stem cells in various human tumors.

Publikationen

Species	Application	Title
human	WB	Front Oncol; USP7 regulates growth and maintains the stemness of p53-mutant colorectal cancer cells via stabilizing of mutant p53; Authors - Xue Li
human,mouse	WB,IF	J Oncol; The Role of Purine Metabolism-Related Genes PPAT and IMPDH1 in the Carcinogenesis of Intrahepatic Cholangiocarcinoma Based on Metabonomic and Bioinformatic Analyses; Authors - Chang-Jun Liu
human	WB	Cancer Med; Integrated analysis based on vesicle trafficking-related genes identifying CNIH4 as a novel therapeutic target for glioma; Authors - Zhen Fang
human	IF	Oncol Lett; Regulation of the migration of colorectal cancer stem cells via the TLR4/MyD88 signaling pathway by the novel surface marker CD14 following LPS stimulation; Authors - Yufei Li
human	WB	World J Oncol; DNA Damage-Induced Apoptosis Suppressor Triggers Progression and Stemness of Glioma by Enhancing Lymphoid Enhancer-Binding Factor 1 Expression; Authors - You Lin Chen
human,mouse	WB	Bioorg Med Chem; Synthesis and anti-tumor activity of menthylamine derivatives: Focusing on the potent inhibitory effect of compound W8 on glioma growth; Authors - Xiaoran Wu