PROS1 Polyklonaler Antikörper

PROS1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16910-1-PBS

Synonyme

PROS, Vitamin K-dependent protein S

---

• 

  WB analysis of human placenta using 16910-1-AP (same clone as 16910-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16910-1-AP (PROS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16910-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16910-1-AP (same clone as 16910-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16910-1-AP (PROS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16910-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 16910-1-AP (same clone as 16910-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16910-1-AP (PROS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16910-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse placenta using 16910-1-AP (same clone as 16910-1-PBS)

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 16910-1-AP (PROS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16910-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16910-1-AP (same clone as 16910-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16910-1-AP (PROS1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16910-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16910-1-AP (same clone as 16910-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16910-1-AP (PROS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16910-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 16910-1-AP (same clone as 16910-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PROS1 antibody (16910-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 16910-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 16910-1-AP (same clone as 16910-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PROS1 (16910-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 16910-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

16910-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA PROS1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PROS1 fusion protein Ag10539
• Vollständiger Name: protein S (alpha)
• Berechnetes Molekulargewicht: 75 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC015801
• Gene symbol: PROS1
• Gene ID (NCBI): 5627
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PROS1, also named as Vitamin K-dependent protein S, is a 676 amino acid protein, which contains 4 EGF-like domains, contains one Gla (gamma-carboxy-glutamate) domain and contains 2 laminin G-like domains. PROS1 as a secreted protein platelets alpha granules is expressed in plasma. PROS1 is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa and helps to prevent coagulation and stimulating fibrinolysis.