PROS1 Rekombinanter Antikörper

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• KD/KO Validated

PROS1 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241241E5

Kat-Nr. : 84107-2-PBS

Synonyme

241241E5, PROS, Vitamin K-dependent protein S

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  WB analysis using 84107-2-RR (same clone as 84107-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84107-2-RR (PROS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84107-2-PBS in a different storage buffer formulation.

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  WB analysis of DU 145 using 84107-2-RR (same clone as 84107-2-PBS)

  WB result of PROS1 antibody (84107-2-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PROS1 transfected DU 145 cells. This data was developed using the same antibody clone with 84107-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01044-1

  Sandwich ELISA standard curve of MP01044-1, Human PROS1 Recombinant Matched Antibody Pair - PBS only. 84107-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag10539. 84107-1-PBS was HRP conjugated as the detection antibody. Range: 0.469-30 ng/mL

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  Sample test of MP01044-1

  The mean PROS1 concentration was determined to be 276.09 ng/mL in HuH-7 cell extract based on a 1.4 mg/mL extract load.

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  IHC staining of mouse brain using 84107-2-RR (same clone as 84107-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84107-2-RR (PROS1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84107-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 84107-2-RR (same clone as 84107-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84107-2-RR (PROS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84107-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84107-2-RR (same clone as 84107-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PROS1 antibody (84107-2-RR, Clone: 241241E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84107-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84107-2-RR (same clone as 84107-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PROS1 antibody (84107-2-RR, Clone: 241241E5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84107-2-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 84107-2-RR (same clone as 84107-2-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug PROS1 Recombinant antibody (84107-2-RR, Clone:241241E5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84107-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01044-1

  Cytometric bead array standard curve of MP01044-1, PROS1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84107-2-PBS. Detection antibody: 84107-1-PBS. Standard: Ag10539. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84107-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84107-2-RR against Human PROS1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

84107-2-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test PROS1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PROS1 fusion protein Ag10539
• Vollständiger Name: protein S (alpha)
• Berechnetes Molekulargewicht: 75 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC015801
• Gene symbol: PROS1
• Gene ID (NCBI): 5627
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PROS1, also named as Vitamin K-dependent protein S, is a 676 amino acid protein, which contains 4 EGF-like domains, contains one Gla (gamma-carboxy-glutamate) domain and contains 2 laminin G-like domains. PROS1 as a secreted protein platelets alpha granules is expressed in plasma. PROS1 is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa and helps to prevent coagulation and stimulating fibrinolysis.