PROSC Rekombinanter Antikörper

PROSC Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240998B6

Kat-Nr. : 83831-5-PBS

Synonyme

PLPBP, 240998B6, PLP homeostasis protein, Proline synthase co-transcribed bacterial homolog protein, Pyridoxal phosphate homeostasis protein

---

• 

  WB analysis using 83831-5-RR (same clone as 83831-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83831-5-RR (PROSC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83831-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 83831-5-RR (same clone as 83831-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83831-5-RR (PROSC antibody) at dilution of 1:650 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83831-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83831-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83831-5-RR against Human PROSC were performed. The affinity constant is 2.00 nM.

---

Geprüfte Anwendungen

Produktinformation

83831-5-PBS bindet in WB, IHC, ELISA PROSC und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PROSC fusion protein Ag18369
• Vollständiger Name: proline synthetase co-transcribed homolog (bacterial)
• Berechnetes Molekulargewicht: 275 aa, 30 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC012334
• Gene symbol: PROSC
• Gene ID (NCBI): 11212
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human Pyridoxal 5′-phosphate-binding protein (PLPBP; previously proline synthase cotranscribed homolog, PROSC) was first described in 1999 and was demonstrated to be ubiquitously expressed in many tissues. PROSC is ubiquitously expressed in human tissues and is highly conserved throughout evolution. PROSC deficiency could therefore markedly increase free cellular PLP levels (PMID: 31825581, 27912044).