PROX1 Monoklonaler Antikörper

PROX1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D4B1

Kat-Nr. : 67438-1-PBS

Synonyme

1D4B1, Homeobox prospero-like protein PROX1, prospero homeobox 1, Prospero homeobox protein 1, PROX-1

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  WB analysis of HuH-7 using 67438-1-Ig (same clone as 67438-1-PBS)

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 67438-1-Ig (PROX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67438-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 67438-1-Ig (same clone as 67438-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67438-1-Ig (PROX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67438-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67438-1-Ig (same clone as 67438-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67438-1-Ig (PROX1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67438-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67438-1-Ig (same clone as 67438-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67438-1-Ig (PROX1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67438-1-PBS in a different storage buffer formulation.

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  IF Staining of HuH-7 using 67438-1-Ig (same clone as 67438-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using PROX1 antibody (67438-1-Ig, Clone: 1D4B1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67438-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67438-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PROX1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PROX1 fusion protein Ag9681
• Vollständiger Name: prospero homeobox 1
• Berechnetes Molekulargewicht: 737 aa, 83 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC024201
• Gene symbol: PROX1
• Gene ID (NCBI): 5629
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PROX1, Prospero homeobox protein 1, belongs to the Prospero homeobox family and contain a Prospero-type homeobox DNA-binding domain. PROX1 plays a fundamental role in early development of CNS and regulates the expression of gene and development of postmitotic, undifferentiated neurons. Also, PROX1 is a required regulator of the development of the lymphatic system. This antibody is raised against part of C-terminal chain of human PROX1. The calculated molecular weight of PROX1 is 83 kDa, but with post-translational modifications, PROX1 is about 90 kDa.