PRPF19 Polyklonaler Antikörper

PRPF19 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 15414-1-AP

Synonyme

EC:2.3.2.27, hPso4, Pre-mRNA-processing factor 19, PRP19, PSO4

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  WB analysis of mouse kidney using 15414-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 15414-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15414-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15414-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15414-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 15414-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 15414-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 15414-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 15414-1-AP (PRPF19 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, HEK-293-Zellen, HeLa-Zellen, humanes Hodengewebe, Mauslebergewebe, PC-3-Zellen, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below

Produktinformation

15414-1-AP bindet in WB, IHC, ELISA PRPF19 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRPF19 fusion protein Ag7700
• Vollständiger Name: PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC008719
• Gene symbol: PRPF19
• Gene ID (NCBI): 27339
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRPF19 is involved in several processes, including DNA damage checkpoint signaling; cellular protein metabolic process; and mRNA splicing, via spliceosome.

Publikationen

Species	Application	Title
mouse	WB	Expert Opin Ther Targets; Proteome of thymus and spleen reveals that 10-hydroxydec-2-enoic acid could enhance immunity in mice.; Authors - Pei Fan
human	WB	Arch Biochem Biophys; RNF113A as a Poor Prognostic Factor Promotes Metastasis and Invasion of Cervical Cancer through miR197/PRP19/P38MAPK Signaling Pathway; Authors - Qingwei Zhang
human	WB	J Cell Biochem; Human RNF113A participates of pre-mRNA splicing in vitro.; Authors - Guilherme H Gatti da Silva
human	WB	iScience; RRM2 promotes liver metastasis of pancreatic cancer by stabilizing YBX1 and activating the TGF-beta pathway; Authors - Zhouyuan Du ; KD Validated
mouse	WB	iScience; Adipocyte-specific Steap4 deficiency reduced thermogenesis and energy expenditure in mice; Authors - Han Wang
pig,human	WB	J Virol; PRPF19 Limits Porcine Epidemic Diarrhea Virus Replication through Targeting and Degrading Viral Capsid Protein; Authors - Xueying Zhai