PRPF3 Polyklonaler Antikörper

PRPF3 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 10106-1-AP

Synonyme

HPRP3, HPRP3P, Pre mRNA splicing factor 3, PRP3, Prp3p, PRPF3, RP18, U4/U6 snRNP 90 kDa protein

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Galerie der Validierungsdaten

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  WB analysis of Y79 using 10106-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 10106-1-AP (PRPF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10106-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10106-1-AP (PRPF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 10106-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10106-1-AP (PRPF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10106-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10106-1-AP (PRPF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10106-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10106-1-AP (PRPF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10106-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10106-1-AP (PRPF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 10106-1-AP

  IP Result of anti-PRPF3 (IP:10106-1-AP, 5ug; Detection:10106-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Y79-Zellen, HepG2-Zellen, L02-Zellen, Maushirngewebe, Mauslebergewebe
• Erfolgreiche IP: Mauslebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

10106-1-AP bindet in WB, IP, IF, ELISA PRPF3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRPF3 fusion protein Ag0154
• Vollständiger Name: PRP3 pre-mRNA processing factor 3 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 78 kDa
• Beobachtetes Molekulargewicht: 90 kDa, 78 kDa
• GenBank-Zugangsnummer: BC000184
• Gene symbol: PRPF3
• Gene ID (NCBI): 9129
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The pre-mRNA splicing occurs in spliceosomes, which consist of four ribonucleoprotein (snRNP) particles (U1, U2, U5 and U4/U6) and more than fifty proteins. This U4/U6-associated splicing factor, Hprp3p, is a 77 kDa protein, which is homologous to the Saccharomyces cerevisiae splicing factor Prp3p and may play a role in spliceosome assembly. Western blot analysis of HeLa cell nuclear extracts detected PRP3 protein at an apparent molecular mass of about 90 kD(PMID: 9328476 ). The discrepancy between the molecular mass reported here and that predicted from the Hprp3p coding sequence can be attributed, at least partially, to the highly positive charge; its value of predicted isoelectric point (IP) is ∼9.99. This antibody is a rabbit polyclonal antibody raised against an internal region of human PRPF3. A predicted molecular mass of 77 kDa and the gene is named PRPF3 because it is homologous to the yeast PRP3 gene. We noticed that although the predicted molecular mass of PRPF3 is77 kDa, the protein produced in E.coli migrated like a 90 kDa protein in SDS-PAGE. (PMID: 9328476)

Publikationen

Species	Application	Title
human	WB	Hum Mol Genet; PRPF mutations are associated with generalized defects in spliceosome formation and pre-mRNA splicing in patients with retinitis pigmentosa.; Authors - Tanackovic Goranka G
human	WB	Mol Cell Biol; A quantitative high-throughput in vitro splicing assay identifies inhibitors of spliceosome catalysis.; Authors - Berg Michael G MG
human	WB	J Biol Chem; PRPF19 regulates p53-dependent cellular senescence by modulating alternative splicing of MDM4 mRNA.; Authors - Kimiyoshi Yano
human	IF	Cell Mol Biol Lett; TMEM43 promotes pancreatic cancer progression by stabilizing PRPF3 and regulating RAP2B/ERK axis.; Authors - Junqiang Li