PRPF31 Polyklonaler Antikörper

PRPF31 Polyklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27750-1-PBS

Synonyme

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  WB analysis using 27750-1-AP (same clone as 27750-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27750-1-AP (PRPF31 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27750-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 27750-1-AP (same clone as 27750-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 27750-1-AP (PRPF31 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27750-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 27750-1-AP (same clone as 27750-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 27750-1-AP (PRPF31 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27750-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse eye using 27750-1-AP (same clone as 27750-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using PRPF31 antibody (27750-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27750-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse eye using 27750-1-AP (same clone as 27750-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using PRPF31 antibody (27750-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27750-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

27750-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA PRPF31 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRPF31 fusion protein Ag26716
• Vollständiger Name: PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: NM_015629
• Gene symbol: PRPF31
• Gene ID (NCBI): 26121
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRPF31 is a component of the spliceosome complex. It is recruited to introns following the attachment of U4 and U6 RNAs and the 15.5K protein. The addition of PRPF31 is crucial for the transition of the spliceosomal complex to the activated state.