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PRPF31 Rekombinanter Antikörper

PRPF31 Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

251005G5

Kat-Nr. : 86315-1-PBS

Synonyme

hPrp31, NY BR 99, Protein 61K, PRP31, RP11

Filter:
  • Western Blot (WB) analysis of various lysates using PRPF31 Recombinant antibody (86315-1-RR)

    WB analysis using 86315-1-RR (same clone as 86315-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86315-1-RR (PRPF31 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86315-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP02586-1

    Sandwich ELISA standard curve of MP02586-1

    Sandwich ELISA standard curve of MP02586-1, Human PRPF31 Recombinant Matched Antibody Pair - PBS only. 86315-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag26716. 86315-3-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Sample test of MP02586-1

    Sample test of MP02586-1

    The mean PRPF31 concentration was determined to be 14.26 ng/mL in HeLa cell extract based on a 1.10 mg/mL extract load.

Geprüfte Anwendungen

Produktinformation

86315-1-PBS bindet in WB, Sandwich ELISA, Indirect ELISA, Sample test PRPF31 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen PRPF31 fusion protein Ag26716
Vollständiger Name PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae)
Berechnetes Molekulargewicht 55 kDa
Beobachtetes Molekulargewicht55-60 kDa
GenBank-ZugangsnummerNM_015629
Gene symbol PRPF31
Gene ID (NCBI) 26121
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRPF31 is a component of the spliceosome complex. It is recruited to introns following the attachment of U4 and U6 RNAs and the 15.5K protein. The addition of PRPF31 is crucial for the transition of the spliceosomal complex to the activated state.