PRPF39 Polyklonaler Antikörper

PRPF39 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 24480-1-AP

Synonyme

Pre mRNA processing factor 39, PRP39 homolog, PRPF39

---

• 

  WB analysis of K-562 using 24480-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 24480-1-AP (PRPF39 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 24480-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 24480-1-AP (PRPF39 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 24480-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 24480-1-AP (PRPF39 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 24480-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24480-1-AP (PRPF39 Antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human testis using 24480-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24480-1-AP (PRPF39 Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 24480-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24480-1-AP (PRPF39 Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 24480-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24480-1-AP (PRPF39 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 24480-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24480-1-AP (PRPF39 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 24480-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24480-1-AP (PRPF39 Antibody) at dilution of 1:400 (under 10x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, COLO 320-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

24480-1-AP bindet in WB, IHC, ELISA PRPF39 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRPF39 fusion protein Ag21683
• Vollständiger Name: PRP39 pre-mRNA processing factor 39 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 669 aa, 78 kDa
• Beobachtetes Molekulargewicht: 75-85 kDa
• GenBank-Zugangsnummer: BC125126
• Gene symbol: PRPF39
• Gene ID (NCBI): 55015
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRPF39 also termed as Pre mRNA processing factor 39 or PRP39 homolog is a 669 amino acid protein, which contains 7 HAT repeats and belongs to the PRP39 family. PRPF39 localizes in nucleus and may play a role in mRNA splicing.

Publikationen

Species	Application	Title
human	WB	Discov Oncol; Prognostic implications of alternative splicing events and key splicing factors in head and neck squamous cell carcinoma; Authors - Siyi He