PRPF4 Polyklonaler Antikörper

PRPF4 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10728-1-AP

Synonyme

HPRP4, HPRP4P, PRP4, PRP4 homolog, Prp4p, PRPF4, U4/U6 snRNP 60 kDa protein, WD splicing factor Prp4

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Galerie der Validierungsdaten

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  WB analysis using 10728-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10728-1-AP (PRPF4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10728-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10728-1-AP (PRPF4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10728-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10728-1-AP (PRPF4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10728-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10728-1-AP (PRPF4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10728-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10728-1-AP (PRPF4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 10728-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10728-1-AP (PRPF4 antibody) at dilution of 1:10 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, K-562-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

10728-1-AP bindet in WB, IHC, ELISA PRPF4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRPF4 fusion protein Ag1193
• Vollständiger Name: PRP4 pre-mRNA processing factor 4 homolog (yeast)
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC001588
• Gene symbol: PRPF4
• Gene ID (NCBI): 9128
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRPF4, also named as U4/U6 small nuclear ribonucleoprotein Prp4, is a 522 amino acid protein, which contains seven WD repeats. PRPF4 localizes in the Nucleus speckle. PRPF4 participates in pre-mRNA splicing and is a part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.PRPF4-mediated phosphorylation of KLF13 plays a role in the regulation of CCL5 expression in T lymphocytes

Publikationen

Species	Application	Title
human	WB	Nucleic Acids Res; MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongation; Authors - Bangming Jin
human	WB	Hum Mol Genet; PRPF mutations are associated with generalized defects in spliceosome formation and pre-mRNA splicing in patients with retinitis pigmentosa.; Authors - Tanackovic Goranka G
mouse	WB	J Biol Chem; Intercellular transfer of proteins as identified by stable isotope labeling of amino acids in cell culture.; Authors - Li Ming M