Peripherin Polyklonaler Antikörper

Peripherin Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 17399-1-AP

Synonyme

peripherin, PRPH, PRPH1

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Galerie der Validierungsdaten

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  WB analysis using 17399-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17399-1-AP (Peripherin antibody) at dilution of 1:120000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 17399-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 17399-1-AP (PRPH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 17399-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 17399-1-AP (PRPH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 17399-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17399-1-AP (Peripherin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 17399-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17399-1-AP (Peripherin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 17399-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17399-1-AP (Peripherin Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 17399-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17399-1-AP (Peripherin Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat brain using 17399-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 17399-1-AP (Peripherin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 17399-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17399-1-AP (PRPH antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17399-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17399-1-AP (PRPH antibody) at dilution of 1:100 (under 40x lens).

• 

  FC experiment of PC-12 using 17399-1-AP

  1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Peripherin (17399-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Kolongewebe, COLO 320-Zellen, humanes Kolongewebe, Ratten-Kolongewebe, Ratten-Rückenmarkgewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Hirngewebe, humanes Kolongewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: PC-12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

17399-1-AP bindet in WB, IHC, IF, FC, ELISA Peripherin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peripherin fusion protein Ag11365
• Vollständiger Name: peripherin
• Berechnetes Molekulargewicht: 470 aa, 54 kDa
• Beobachtetes Molekulargewicht: 54-60 kDa
• GenBank-Zugangsnummer: BC032703
• Gene symbol: PRPH
• Gene ID (NCBI): 5630
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Peripherin is a neuron-specific intermediate filament protein that is particularly expressed in peripheral nervous system. It is used to study peripheral nerve development and regeneration, as it is highly expressed during elongation of axon. Peripherin is a routine marker for unmyelinated neurons in trigeminal ganglion (TG) and the dorsal root ganglion (DRG). Peripherin is also expressed in peri-insular areas of islets and it has been identified as a key autoantigen for type 1A diabetes.

Publikationen

Species	Application	Title
rat	IF	Biotechniques; Antibody incubation at 37°C improves fluorescent immunolabeling in free-floating thick tissue sections.; Authors - Xia Xiao