PRPH2 Rekombinanter Antikörper

PRPH2 Rekombinant Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

242170F4

Kat-Nr. : 85043-1-RR

Synonyme

242170F4, AOFMD, AVMD, Peripherin 2, Peripherin2

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  WB analysis using 85043-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85043-1-RR (PRPH2 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse eye using 85043-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85043-1-RR (PRPH2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse eye using 85043-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85043-1-RR (PRPH2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse eye using 85043-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PRPH2 antibody (85043-1-RR, Clone: 242170F4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse eye using 85043-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PRPH2 antibody (85043-1-RR, Clone: 242170F4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Augengewebe, Ratten-Augengewebe
• Erfolgreiche Detektion in IHC: Maus-Augengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Augengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)-P: IF-P : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85043-1-RR bindet in WB, IHC, IF-P, ELISA PRPH2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PRPH2 fusion protein Ag12555
• Vollständiger Name: peripherin 2 (retinal degeneration, slow)
• Berechnetes Molekulargewicht: 346 aa, 39 kDa
• Beobachtetes Molekulargewicht: 30-40 kDa
• GenBank-Zugangsnummer: BC074720
• Gene symbol: PRPH2
• Gene ID (NCBI): 5961
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Peripherin-2 (PRPH2), also known as retinal degeneration slow protein (RDS), is a photoreceptor-specific tetraspanin protein implicated in outer segment disk morphogenesis. It may function as an adhesion molecule involved in the stabilization and compaction of outer segment disks or in the maintenance of the curvature of the rim. Mutations in peripherin-2 are responsible for various retinal degenerative diseases including autosomal dominant retinitis pigmentosa (ADRP).