PRPS1 Polyklonaler Antikörper

PRPS1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(17)

Kat-Nr. : 15549-1-AP

Synonyme

PRS I, PPRibP, KIAA0967, EC:2.7.6.1, CMTX5

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  WB analysis using 15549-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15549-1-AP (PRPS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 15549-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 15549-1-AP (PRPS1 Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15549-1-AP

  IP result of anti-PRPS1 (IP:15549-1-AP, 4ug; Detection:15549-1-AP 1:2000) with HeLa cells lysate 1600 ug.

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  IHC staining of mouse testis using 15549-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15549-1-AP (PRPS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 15549-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15549-1-AP (PRPS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U-251 using 15549-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using PRPS1 antibody (15549-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, Mausnierengewebe, Rattennierengewebe, Rattenmilzgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 12 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• CoIP: See 2 publications below

Produktinformation

15549-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA PRPS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRPS1 fusion protein Ag7907
• Vollständiger Name: phosphoribosyl pyrophosphate synthetase 1
• Berechnetes Molekulargewicht: 318 aa, 35 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC001605
• Gene symbol: PRPS1
• Gene ID (NCBI): 5631
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRPS1, also named as Ribose-phosphate pyrophosphokinase 1, is a 318 amino acid protein, which belongs to the ribose-phosphate pyrophosphokinase family. PRPS1 can form Homodimer and the active form is probably a hexamer composed of 3 homodimers. PRPS1 catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) which is essential for nucleotide synthesis. PRPS1 as a module has an association with the Hepatocellular carcinoma.

Publikationen

Species	Application	Title
		Science; mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.; Authors - Issam Ben-Sahra
human	CoIP,WB	Sci Adv; NOTCH1-driven UBR7 stimulates nucleotide biosynthesis to promote T cell acute lymphoblastic leukemia.; Authors - Shashank Srivastava
human	IHC	J Invest Dermatol; Enhanced Glycogen Metabolism Supports the Survival and Proliferation of HPV-Infected Keratinocytes in Condylomata Acuminata.; Authors - Zhichao Gu
human	WB	Oncotarget; CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.; Authors - Zhichao Gu
human	WB, IHC	Front Cell Dev Biol; Thyroid Hormone-Regulated Expression of Period2 Promotes Liver Urate Production.; Authors - Xiaoting Chen
human	WB	Amino Acids; Quantitative proteomic dissection of a native 14-3-3ε interacting protein complex associated with hepatocellular carcinoma.; Authors - Chen Bai