PRPS2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PRPS2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 27024-1-AP

Synonyme

Ribose-phosphate pyrophosphokinase 2, PRS-II, PRSII, PRS II, PPRibP

---

• 

  WB analysis of A375 using 27024-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 27024-1-AP (PRPS2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 27024-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 27024-1-AP (PRPS2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen using 27024-1-AP

  IP result of anti-PRPS2 (IP:27024-1-AP, 4ug; Detection:27024-1-AP 1:1000) with mouse spleen tissue lysate 1560 ug.

• 

  IP experiment of A375 using 27024-1-AP

  IP result of anti-PRPS2 (IP:27024-1-AP, 4ug; Detection:27024-1-AP 1:1000) with A375 cells lysate 1600 ug.

• 

  IHC staining of human stomach using 27024-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27024-1-AP (PRPS2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A375 using 27024-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A375 cells using PRPS2 antibody (27024-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen
• Erfolgreiche IP: A375-Zellen, Mausmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A375-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

27024-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA PRPS2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRPS2 fusion protein Ag25785
• Vollständiger Name: phosphoribosyl pyrophosphate synthetase 2
• Beobachtetes Molekulargewicht: 30-34 kDa
• GenBank-Zugangsnummer: BC119662
• Gene symbol: PRPS2
• Gene ID (NCBI): 5634
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRPS (phosphoribosyl pyrophosphate synthetase) proteins catalyze the synthesis of phosphoribosyl pyrophosphate (PRPP). Three human PRPS isoforms exist and are encoded by three different genes. PRPS1 and PRPS2 (also known as PRS1 and PRS2, respectively) are ubiquitously expressed, while PRPS3 (also known as PRPS1L1) is specific to the testis. PRPP is an important substrate synthesized from MgATP and ribose-5-phosphate in a reaction that requires inorganic phosphate and magnesium as a cofactor. PRPP is essential in the synthesis of nearly all nucleotides, implying that PRPS proteins play an important role in nucleotide biosynthesis and purine metabolism. PRPS2 is a 318 amino acid protein that exists as a homodimer and a hexamer composed of three homodimers.

Publikationen

Species	Application	Title
human	CoIP,WB	Sci Adv; NOTCH1-driven UBR7 stimulates nucleotide biosynthesis to promote T cell acute lymphoblastic leukemia.; Authors - Shashank Srivastava
human	WB, IHC	PLoS Biol; Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway.; Authors - Yajing Lv ; KD Validated
human	WB, IHC	Front Cell Dev Biol; Thyroid Hormone-Regulated Expression of Period2 Promotes Liver Urate Production.; Authors - Xiaoting Chen
human	WB	Nat Metab; Mitochondrial NADP+ is essential for proline biosynthesis during cell growth.; Authors - Diem H Tran
human	WB	Nat Commun; Identification of purine biosynthesis as an NADH-sensing pathway to mediate energy stress; Authors - Ronghui Yang ; KD Validated
human,mouse	WB	Cell; De novo and salvage purine synthesis pathways across tissues and tumors; Authors - Diem H Tran