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PRPS2 Rekombinanter Antikörper

PRPS2 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242657G2

Kat-Nr. : 85090-5-PBS

Synonyme

242657G2, EC:2.7.6.1, PPRibP, PRS II, PRSII

Filter:
  • Western Blot (WB) analysis of various lysates using PRPS2 Recombinant antibody (85090-5-RR)

    WB analysis using 85090-5-RR (same clone as 85090-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85090-5-RR (PRPS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85090-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat spleen tissue using PRPS2 Recombinant antibody (85090-5-RR)

    WB analysis of rat spleen using 85090-5-RR (same clone as 85090-5-PBS)

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 85090-5-RR (PRPS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85090-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PRPS2 Recombinant antibody (85090-5-RR)

    IF Staining of HeLa using 85090-5-RR (same clone as 85090-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRPS2 antibody (85090-5-RR, Clone: 242657G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85090-5-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

85090-5-PBS bindet in WB, IF/ICC, Indirect ELISA PRPS2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen PRPS2 fusion protein Ag25785
Vollständiger Name phosphoribosyl pyrophosphate synthetase 2
Beobachtetes Molekulargewicht 30-34 kDa
GenBank-ZugangsnummerBC119662
Gene symbol PRPS2
Gene ID (NCBI) 5634
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRPS (phosphoribosyl pyrophosphate synthetase) proteins catalyze the synthesis of phosphoribosyl pyrophosphate (PRPP). Three human PRPS isoforms exist and are encoded by three different genes. PRPS1 and PRPS2 (also known as PRS1 and PRS2, respectively) are ubiquitously expressed, while PRPS3 (also known as PRPS1L1) is specific to the testis. PRPP is an important substrate synthesized from MgATP and ribose-5-phosphate in a reaction that requires inorganic phosphate and magnesium as a cofactor. PRPP is essential in the synthesis of nearly all nucleotides, implying that PRPS proteins play an important role in nucleotide biosynthesis and purine metabolism. PRPS2 is a 318 amino acid protein that exists as a homodimer and a hexamer composed of three homodimers.