PRPSAP2 Polyklonaler Antikörper

PRPSAP2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 17814-1-AP

Synonyme

PAP41, PRPSAP2

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  WB analysis using 17814-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17814-1-AP (PRPSAP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17814-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17814-1-AP (PRPSAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 17814-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 17814-1-AP (PRPSAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 17814-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 17814-1-AP (PRPSAP2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human pancreas using 17814-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 17814-1-AP (PRPSAP2 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of BxPC-3 using 17814-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using PRPSAP2 antibody (17814-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, HeLa-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: BxPC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

17814-1-AP bindet in WB, IHC, IF/ICC, ELISA PRPSAP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRPSAP2 fusion protein Ag12132
• Vollständiger Name: phosphoribosyl pyrophosphate synthetase-associated protein 2
• Berechnetes Molekulargewicht: 369 aa, 41 kDa
• Beobachtetes Molekulargewicht: 37-41 kDa
• GenBank-Zugangsnummer: BC106050
• Gene symbol: PRPSAP2
• Gene ID (NCBI): 5636
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRPSAP2, also named as PAP41, is 369 amino acid protein, which Seems to play a negative regulatory role in 5-phosphoribose 1-diphosphate synthesis. PRPSAP2 is another protein involved in nucleic acid metabolism. The PRPSAP2 gene encodes part of the enzyme PRPP synthetase, which catalyzes the formation of phosphoribosylpyrophosphate, being a primary substrate for newly formed purine and pyrimidine nucleotides. Depletion of the PRPP synthetase causes growth arrest], the reverse has not been shown. Overexpression of SHMT1 and PRPSAP2 (and also COPS3) has been reported to occur in multiple myelomas . PRPSAP2 exists several isoforms with the range of molecular weight about 35-43 kDa.

Publikationen

Species	Application	Title
mouse	WB	bioRxiv; Evolutionary origins and innovations sculpting the mammalian PRPS enzyme complex; Authors - Bibek R Karki ; KO Validated
human	WB	bioRxiv; PRPS activity tunes redox homeostasis in Myc-driven lymphoma; Authors - Austin C MacMillan