PR3/PRTN3 Polyklonaler Antikörper

PR3/PRTN3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27153-1-AP

Synonyme

PRTN3, PR3, NP-4, Myeloblastin, MBN

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  WB analysis of human spleen using 27153-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 27153-1-AP (PRTN3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 27153-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 27153-1-AP (PRTN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human spleen using 27153-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 27153-1-AP (PRTN3 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 27153-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 27153-1-AP (PRTN3 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 27153-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27153-1-AP (PRTN3 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 27153-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27153-1-AP (PRTN3 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 27153-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27153-1-AP (PRTN3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 27153-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27153-1-AP (PRTN3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Milzgewebe, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Milzgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

27153-1-AP bindet in WB, IHC, ELISA PR3/PRTN3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PR3/PRTN3 fusion protein Ag25915
• Vollständiger Name: proteinase 3
• Berechnetes Molekulargewicht: 256 aa, 28 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC096183
• Gene symbol: PRTN3
• Gene ID (NCBI): 5657
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.