PRUNE Polyklonaler Antikörper

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• KD/KO Validated

PRUNE Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18537-1-PBS

Synonyme

Exopolyphosphatase PRUNE1, EC:3.6.1.1, Drosophila-related expressed sequence 17, DRES-17, DRES17

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  WB analysis using 18537-1-AP (same clone as 18537-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18537-1-AP (PRUNE antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18537-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 18537-1-AP (same clone as 18537-1-PBS)

  WB result of PRUNE antibody (18537-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRUNE transfected HepG2 cells. This data was developed using the same antibody clone with 18537-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 18537-1-AP (same clone as 18537-1-PBS)

  IP result of anti-PRUNE (IP:18537-1-AP, 3ug; Detection:18537-1-AP 1:500) with HepG2 cells lysate 1720ug. This data was developed using the same antibody clone with 18537-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 18537-1-AP (same clone as 18537-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18537-1-AP (PRUNE antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18537-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 18537-1-AP (same clone as 18537-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18537-1-AP (PRUNE antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18537-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 18537-1-AP (same clone as 18537-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PRUNE antibody (18537-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 18537-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18537-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA PRUNE und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRUNE fusion protein Ag13275
• Vollständiger Name: prune homolog (Drosophila)
• Berechnetes Molekulargewicht: 453 aa, 50 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC025304
• Gene symbol: PRUNE
• Gene ID (NCBI): 58497
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.