PSAP Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PSAP Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (3)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(22)

Rezensionen (2)

Kat-Nr. : 10801-1-AP

Synonyme

Dispersin, CSAct, Component C, Co beta glucosidase, Cerebroside sulfate activator

---

• 

  WB analysis using 10801-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10801-1-AP (PSAP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10801-1-AP

  WB result of PSAP antibody (10801-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAP transfected HepG2 cells.

• 

  IHC staining of human breast cancer using 10801-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10801-1-AP (PSAP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10801-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10801-1-AP (PSAP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 10801-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10801-1-AP (PSAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 10801-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 10801-1-AP (PSAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U-251 using 10801-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PSAP antibody (10801-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 10801-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSAP antibody (10801-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of HEK-293 using 10801-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 10801-1-AP (PSAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, Maus-Cerebellum-Gewebe, Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HEK-293-Zellen, U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 19 publications below
• IHC: See 3 publications below
• IF: See 7 publications below
• ELISA: See 2 publications below
• FC: See 1 publications below

Produktinformation

10801-1-AP bindet in WB, IHC, IF/ICC, ELISA PSAP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSAP fusion protein Ag1194
• Vollständiger Name: prosaposin
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC001503
• Gene symbol: PSAP
• Gene ID (NCBI): 5660
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The PSAP gene encodes prosaposin, a precursor of four small nonenzymatic glycoproteins termed 'sphingolipid activator proteins' (SAPs) that assist in the lysosomal hydrolysis of sphingolipids. After proteolytic processing of the presaposin protein, these 4 released polypeptides are functional activators. Saposin A is encoded by residues 60 to 143 of PSAP, saposin B by 195 to 275, saposin C by 311 to 390, and saposin D by 405 to 487. There are four 12-14 kDa heatstable glycoproteins. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. Saposins A-D are required for the hydrolysis of certain sphingolipids by specific lysosomal hydrolases. (PMID: 2001789) Defects in PSAP are the cause of Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy (PubMed: 2060627, PMID: 15773042). This PSAP antibody (10801-1-AP) is expected to recognize both saposin A and B.

Publikationen

Species	Application	Title
human	IF,WB	Nat Neurosci; Genome-wide CRISPRi/a screens in human neurons link lysosomal failure to ferroptosis.; Authors - Ruilin Tian
human	IF	Adv Sci (Weinh); Integrative Transcriptomic Analyses of Hippocampal-Entorhinal System Subfields Identify Key Regulators in Alzheimer's Disease; Authors - Dan Luo
human	WB	Nat Commun; Prosaposin is a regulator of progranulin levels and oligomerization.; Authors - Alexandra M Nicholson ; KD Validated
human	WB	Nat Commun; Impaired prosaposin lysosomal trafficking in frontotemporal lobar degeneration due to progranulin mutations.; Authors - Xiaolai Zhou
mouse	WB	Mol Neurodegener; Accumulation of saposin in dystrophic neurites is linked to impaired lysosomal functions in Alzheimer's disease brains.; Authors - Md Golam Sharoar
mouse	WB	Proc Natl Acad Sci U S A; Inhibition of sphingolipid synthesis improves outcomes and survival in GARP mutant wobbler mice, a model of motor neuron degeneration.; Authors - Constance S Petit

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Sharan (Verified Customer) (05-28-2020)

The antibody works well in western blot assays with minimum cross-reactivity

• Applications: Western Blot

PSAP Antibody Western Blot validation ( dilution) in (Cat no:10801-1-AP)

FH

Krishnendu (Verified Customer) (02-27-2020)

Very specific for AIMP3

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
• Primary Antibody Dilution: 1:2000 for WB, 1:100 For IP and 1:250 for IF
• Cell Tissue Type: HEK293T, MCF7