PSAP Monoklonaler Antikörper

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• KD/KO Validated

PSAP Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2F11B2

Kat-Nr. : 66473-1-PBS

Synonyme

CSAct, Component C, Co beta glucosidase, Cerebroside sulfate activator, A1 activator

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  WB analysis of human milk using 66473-1-Ig (same clone as 66473-1-PBS)

  human milk were subjected to SDS PAGE followed by western blot with 66473-1-Ig (PSAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66473-1-Ig (same clone as 66473-1-PBS)

  WB result of PSAP antibody (66473-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAP transfected HeLa cells. This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66473-1-Ig (same clone as 66473-1-PBS)

  WB result of PSAP antibody (66473-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAP transfected HepG2 cells. This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66473-1-Ig (same clone as 66473-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66473-1-Ig (PSAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 66473-1-Ig (same clone as 66473-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66473-1-Ig (PSAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66473-1-Ig (same clone as 66473-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66473-1-Ig (PSAP antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66473-1-Ig (same clone as 66473-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66473-1-Ig (PSAP antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

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  IF Staining of human prostate cancer using 66473-1-Ig (same clone as 66473-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using PSAP antibody (66473-1-Ig, Clone: 2F11B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

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  IF Staining of human prostate cancer using 66473-1-Ig (same clone as 66473-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using PSAP antibody (66473-1-Ig, Clone: 2F11B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66473-1-Ig (same clone as 66473-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSAP antibody (66473-1-Ig, Clone: 2F11B2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66473-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA PSAP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PSAP fusion protein Ag1194
• Vollständiger Name: prosaposin
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC001503
• Gene symbol: PSAP
• Gene ID (NCBI): 5660
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.