PSAT1 Monoklonaler Antikörper

PSAT1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

1B2C9

Rezensionen (1)

Kat-Nr. : 67619-1-Ig

Synonyme

1B2C9, EC:2.6.1.52, EPIP, Phosphoserine aminotransferase, PSA

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• 

  WB analysis using 67619-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67619-1-Ig (PSAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  IHC staining of human breast cancer using 67619-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67619-1-Ig (PSAT1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HCT 116 using 67619-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using PSAT1 antibody (67619-1-Ig, Clone: 1B2C9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, K-562-Zellen, Maushirngewebe, NIH/3T3-Zellen, Hausschwein-Hirngewebe, Kaninchenhirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HCT 116-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Produktinformation

67619-1-Ig bindet in WB, IHC, IF/ICC, ELISA PSAT1 und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PSAT1 fusion protein Ag14076
• Vollständiger Name: phosphoserine aminotransferase 1
• Berechnetes Molekulargewicht: 370 aa, 40 kDa
• Beobachtetes Molekulargewicht: 37-40 kDa
• GenBank-Zugangsnummer: BC001618
• Gene symbol: PSAT1
• Gene ID (NCBI): 29968
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSAT1, also named as PSA and PSAT, belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family and SerC subfamily. It catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine. PSAT1 represents a new interesting target for CRC therapy. (PMID:18221502) It may be implicated in altered serine metabolism and schizophrenia spectrum conditions. (PMID:20955740 )

Publikationen

Species	Application	Title
human	WB,IHC	Thorac Cancer; Exosomal ERBB2IP contributes to tumor growth via elevating PSAT1 expression in non-small cell lung carcinoma; Authors - Xijuan Peng
human	IHC	Cancer Biother Radiopharm; Prognostic Signature in Osteosarcoma Based on Amino Acid Metabolism-Associated Genes; Authors - Liwen Feng
human	WB	Exp Mol Med; The m6A writer RBM15 drives the growth of triple-negative breast cancer cells through the stimulation of serine and glycine metabolism; Authors - Su Hwan Park
human	WB	Cancer Metab; Serine starvation suppresses the progression of esophageal cancer by regulating the synthesis of purine nucleotides and NADPH; Authors - Hui Jie ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (01-17-2023)

Good antibody, however the actual results are not as good as that showing on the website. I have to use much lower dilution as 1:1000.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hela, Mouse liver and mouse brain

PSAT1 Antibody Western Blot validation (1:1000 dilution) in Hela, Mouse liver and mouse brain (Cat no:67619-1-Ig)